Recombinant Mouse CD74 Protein, CF

R&D Systems | Catalog # 7478-CD

R&D Systems
Discontinued Product
7478-CD has been discontinued. View all CD74 products.

Key Product Details

Source

CHO

Accession Number

NP_034675

Applications

Bioactivity
View all CD74 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived mouse CD74 protein
Gln56-Leu215, with an N-terminal HA-tag (YPYDVPDYA)

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Tyr

Predicted Molecular Mass

19.4 kDa

SDS-PAGE

26-40 kDa, reducing conditions

Activity

Measured by the ability of the immobilized protein to support the adhesion of HeLa human cervical epithelial carcinoma cells.
When 5 x 104 cells per well are added to Recombinant Mouse CD74 coated plates, cell adhesion is enhanced in a dose dependent manner. The ED50 for this effect is 0.3-1.5 μg/mL.
Optimal dilutions should be determined by each laboratory for each application.

Formulation, Preparation, and Storage

7478-CD
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CD74

CD74, also known as Invariant chain (Ii) and p33, is a type 2 transmembrane glycoprotein that plays an important role in adaptive immunity, inflammation, and cancer (1). Mature mouse CD74 consists of a 29 amino acid (aa) cytoplasmic domain, a 29 aa transmembrane segment, and a 224 aa extracellular domain (ECD) that contains one thyroglobulin type 1 domain (2). Alternate splicing generates a short isoform that lacks the thyroglobulin domain (2). Within the ECD, mouse CD74 shares 75% and 88% aa sequence identity with human and rat CD74, respectively. CD74 functions as a chaperone for MHC class II molecules on antigen presenting cells and undergoes progressive proteolysis during class II trafficking and antigenic peptide loading (3). Full length CD74 assembles into trimers which then associate with class II molecules in nonameric complexes on the cell surface (4, 5). CD74 also associates with CD44 and binds with high affinity to the cytokine MIF, leading to inflammatory leukocyte responses, protection from tissue fibrosis, B cell proliferative and survival signaling, and the up‑regulation of angiogenic factors in endometrial stromal cells (6‑11). MIF binding notably induces the proteolytic cleavage of the CD74 intracellular domain which then promotes B cell differentiation (10). CD74 is up‑regulated on non‑immune cells at sites of inflammation including amyloid beta plaques and atherosclerotic plaques (12, 13). It is also up‑regulated in a variety of cancers and enhances tumorigenicity, tumor angiogenesis, and metastasis (1, 14).

References

  1. Beswick, E.J. and V.E. Reyes (2009) World J. Gastroenterol. 15:2855.
  2. Koch, N. et al. (1987) EMBO J. 6:1677.
  3. Riberdy, J.M. et al. (1992) Nature 360:474.
  4. Koch, N. et al. (1991) J. Immunol. 147:2643.
  5. Roche, P.A. et al. (1991) Nature 354:392.
  6. Leng, L. et al. (2003) J. Exp. Med. 197:1467.
  7. Takahashi, K. et al. (2009) Respir. Res. 10:33.
  8. Heinrichs, D. et al. (2011) Proc. Natl. Acad. Sci. 104:17444.
  9. Shi, X. et al. (2006) Immunity 25:595.
  10. Gore, Y. et al. (2008) J. Biol. Chem. 283:2784.
  11. Veillat, V. et al. (2010) J. Clin. Endocrinol. Metab. 95:E403.
  12. Bryan, K.J. et al. (2008) Mol. Neurodegen. 3:13.
  13. Martin-Ventura, J.L. et al. (2009) Cardiovasc. Res. 83:586.
  14. Liu, Y.-H. et al. (2008) J. Immunol. 181:6584.

Alternate Names

CD74, CLIP, DHLAG, HLADG, Ia-gamma, INVG34

Entrez Gene IDs

972 (Human); 16149 (Mouse); 25599 (Rat)

Gene Symbol

CD74

UniProt

NP_034675

Additional CD74 Products

Product Documents for Recombinant Mouse CD74 Protein, CF

Certificate of Analysis

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Product Specific Notices for Recombinant Mouse CD74 Protein, CF

For research use only

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