Recombinant Mouse Galectin-1 Protein

Carrier Free

Catalog # Availability Size / Price Qty
1245-GA-050/CF

With Carrier

Catalog # Availability Size / Price Qty
1245-GA-050
R&D Systems Recombinant Proteins and Enzymes
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Citations (12)
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Recombinant Mouse Galectin-1 Protein Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to agglutinate human red blood cells. Hadari, Y.R. et al. (2000) J. Cell Sci. 113:2385. The ED50 for this effect is 1-5 µg/mL.
Source
E. coli-derived mouse Galectin-1 protein
Ala2-Glu135
Accession #
N-terminal Sequence
Analysis
Ala2
Predicted Molecular Mass
15 kDa

Product Datasheets

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1245-GA (with carrier)

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1245-GA/CF (carrier free)

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

1245-GA

Formulation Lyophilized from a 0.2 μm filtered solution in PBS, EDTA and DTT with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

1245-GA/CF

Formulation Lyophilized from a 0.2 μm filtered solution in PBS, EDTA and DTT.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

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Background: Galectin-1

Galectin-1, gene name LGALS1 (lectin, galactoside-binding, soluble 1), is a 135 amino acid (aa), 14 kDa, pleiotropic, non-glycosylated, monomeric or homodimeric carbohydrate-binding protein of the prototype galectin family (1-3). Galectins lack a classical signal peptide and can be localized to the cytosolic compartments, or secreted via non-classical pathways (1). Secreted Galectin-1 has immunosuppressive and anti-inflammatory properties and suppresses acute and chronic inflammation and autoimmunity. It contributes to negative selection of developing T cells, immunosuppression by regulatory T cells, resolution of the inflammatory response, and inhibition of immune cell migration, inflammatory cytokine production, and mast cell degranulation (1, 2, 4-6). Galectin-1 preferentially binds laminin, fibronectin, 90K/Mac-2BP, CD45, CD43, CD7, CD2, CD3, integrins alpha 4 beta 1, alpha 5 beta 1 and alpha 4 beta 7, and ganglioside GM1 (2, 3). It is produced in a variety of tissues by cells that include endothelial cells, connective tissue fibroblasts, thymic stromal cells, tumor cells, muscle cells, platelets, regulatory T cells, and activated tissue macrophages, B cells, T cells and dendritic cells (2, 3, 6-11). Most of this expression is cytosolic. Mouse Galectin-1 shares 88% aa sequence identity with human, 96% with rat, and 84% with equine, ovine, bovine and porcine Galectin-1.  Endothelial cell surface expression, including tumor endothelial cells, is greatly increased by cell activation (9). Galectin-1 is highly expressed at the maternal-fetal interface and contributes to fetal immune privilege (5, 12). Its immunosuppressive properties appear to also allow tumor cells to evade immune detection (4, 5). It selectively controls T cell survival by inducing apoptosis of activated Th1 and Th17 cells, which express Galectin‑1‑binding glycans, while promoting Th2 cell survival where glycans are sialylated and less recognized (4, 13). It also induces apoptosis of immature thymocytes (3, 6). Galectin-1 secreted from bone marrow stromal cells aids B lymphocyte development by contributing to pre-B cell integrin adhesion and receptor signaling (3). The dimer form of Galectin-1 also induces neutrophil down‑regulation by inducing cell surface exposure of phosphatidylserine that marks the cell for phagocytosis (14). Galectin-1 can also modulate cell-cell and cell-matrix interactions, and can promote either cell attachment or detachment depending on the cell type and developmental stage (1, 2).

References
  1. Di Lella, S. et al. (2011) Biochemistry 50:7842.
  2. Rabinovich, G.A. and J.M. Ilarregui (2009) Immunol. Rev. 230:144.
  3. Rabinovich, G.A. and M. Vidal. (2011) Curr. Opin. Hematol. 18:443.
  4. Cedeno-Laurent, F. and C.J. Dimitroff (2012) Clin. Immunol. 142:107.
  5. Than, N.G. et al. (2012) Trends Endocrinol. Metab. 23:23.
  6. Rubinstein, N. et al. (2004) Tissue Antigens 64:1.
  7. Courad, P.O. et al. (1989) J. Biol. Chem. 264:1310.
  8. Hirabayashi, J. et al. (1989) Biochim. Biophys. Acta 1008:85.
  9. Thijssen, V.L. et al. (2008) Am. J. Pathol. 172:545.
  10. Nio-Kobayashi, J. et al. (2009) J. Histochem. Cytochem. 57:41.
  11. Dias-Baruffi, M. et al. (2010) Glycobiology 20:507.
  12. Than, N.G. et al. (2008) Proc. Natl. Acad. Sci. USA 105:15819.
  13. Toscano, M.A. et al. (2007) Nat. Immunol. 8:825.
  14. Stowell, S.R. et al. (2009) J. Biol. Chem. 284:4989.
Entrez Gene IDs
3956 (Human); 16852 (Mouse)
Alternate Names
14 kDa laminin-binding protein; 14 kDa lectin; Beta-galactoside-binding lectin L-14-I; BHL; DKFZp686E23103; GAL1; gal-1; Galaptin; galectin 1; Galectin1; Galectin-1; GBP; HBL; HLBP14; HPL; L-14; Lactose-binding lectin 1; Lectin galactoside-binding soluble 1; lectin, galactoside-binding, soluble, 1; LGALS1; Putative MAPK-activating protein PM12; S-Lac lectin 1

Citations for Recombinant Mouse Galectin-1 Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

12 Citations: Showing 1 - 10
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  1. Dying transplanted neural stem cells mediate survival bystander effects in the injured brain
    Authors: W Han, EM Mei beta ner, S Neunteibl, M Günther, J Kahnt, A Dolga, C Xie, N Plesnila, C Zhu, K Blomgren, C Culmsee
    Cell Death & Disease, 2023-03-01;14(3):173.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  2. Control of intestinal inflammation by glycosylation-dependent lectin-driven immunoregulatory circuits
    Authors: LG Morosi, AM Cutine, AJ Cagnoni, MN Manselle-C, DO Croci, JP Merlo, RM Morales, M May, JM Pérez-Sáez, MR Girotti, SP Méndez-Hue, B Pucci, AH Gil, SP Huernos, GH Docena, AM Sambuelli, MA Toscano, GA Rabinovich, KV Mariño
    Science Advances, 2021-06-18;7(25):.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: ELISA Standard
  3. Hepatic stellate cells contribute to liver regeneration through galectins in hepatic stem cell niche
    Authors: JY Ge, YW Zheng, T Tsuchida, K Furuya, H Isoda, H Taniguchi, N Ohkohchi, T Oda
    Stem Cell Res Ther, 2020-09-29;11(1):425.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  4. Galectin-1 drives lymphoma CD20 immunotherapy resistance: validation of a preclinical system to identify resistance mechanisms
    Authors: JM Lykken, M Horikawa, V Minard-Col, M Kamata, T Miyagaki, JC Poe, TF Tedder
    Blood, 2016-02-17;127(15):1886-95.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  5. Galectin-9 controls the therapeutic activity of 4-1BB-targeting antibodies.
    Authors: Madireddi S, Eun S, Lee S, Nemcovicova I, Mehta A, Zajonc D, Nishi N, Niki T, Hirashima M, Croft M
    J Exp Med, 2014-06-23;211(7):1433-48.
    Species: Mouse
    Sample Types: Protein
    Applications: Bioassay
  6. Induction of galectin-1 by TGF-beta1 accelerates fibrosis through enhancing nuclear retention of Smad2.
    Authors: Lim M, Ahn J, Yi J, Kim M, Son A, Lee S, Lim D, Kim S, Kang M, Han Y, Song J
    Exp Cell Res, 2014-06-11;326(1):125-35.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  7. Galectin-1 attenuates astrogliosis-associated injuries and improves recovery of rats following focal cerebral ischemia.
    Authors: Qu WS, Wang YH, Ma JF, Tian DS, Zhang Q, Pan DJ, Yu ZY, Xie MJ, Wang JP, Wang W
    J. Neurochem., 2010-12-02;116(2):217-26.
    Species: Rat
    Sample Types: In Vivo, Whole Cells
    Applications: Bioassay, In Vivo
  8. Development of a nascent galectin-1 chimeric molecule for studying the role of leukocyte galectin-1 ligands and immune disease modulation.
    Authors: Cedeno-Laurent F, Barthel SR, Opperman MJ, Lee DM, Clark RA, Dimitroff CJ
    J. Immunol., 2010-09-15;185(8):4659-72.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  9. Galectin-1 promotes immunoglobulin production during plasma cell differentiation.
    Authors: Tsai CM, Chiu YK, Hsu TL, Lin IY, Hsieh SL, Lin KI
    J. Immunol., 2008-10-01;181(7):4570-9.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  10. Identification of a lectin causing the degeneration of neuronal processes using engineered embryonic stem cells.
    Authors: Plachta N, Annaheim C, Bissiere S, Lin S, Ruegg M, Hoving S, Muller D, Poirier F, Bibel M, Barde YA
    Nat. Neurosci., 2007-05-07;10(6):712-9.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  11. Altered expression of galectin-3 induces cortical thymocyte depletion and premature exit of immature thymocytes during Trypanosoma cruzi infection.
    Authors: Silva-Monteiro E, Reis Lorenzato L, Kenji Nihei O, Junqueira M, Rabinovich GA, Hsu DK, Liu FT, Savino W, Chammas R, Villa-Verde DM
    Am. J. Pathol., 2007-02-01;170(2):546-56.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  12. Galectin-1 supports survival of naive T cells without promoting cell proliferation.
    Authors: Endharti AT, Zhou YW, Nakashima I, Suzuki H
    Eur. J. Immunol., 2005-01-01;35(1):86-97.
    Species: Mouse
    Sample Types: N/A, Whole Cells
    Applications: Bioassay, Western Blot

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