Recombinant Mouse Integrin alpha V beta 8 Protein, CF Summary
|Mouse Integrin aV
Accession # P43406
|His-Pro||GGGSGGGS||Acidic Tail||6-His tag|
|Mouse Integrin beta 8
Accession # Q0VBD0
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CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in PBS and Trehalose.|
|Reconstitution||Reconstitute at 500 μg/mL in sterile PBS.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Background: Integrin alpha V beta 8
Integrin alpha V beta 8 is a type I transmembrane non-covalent heterodimer composed of a 115 kDa alpha V/CD51 subunit and an 84 kDa beta 8 subunit. The Integrin beta 8 exclusively partners with the alpha V subunit, while alpha V forms heterodimers with beta 1, beta 3, beta 5 and beta 6 (1). Integrin alpha V beta 8 is expressed on Schwann cells and astrocytes in the brain, vascular epithelial cells, mesangial cells in the kidney, and fibroblasts and epithelial cells in the airway (2-7). Interestingly, Integrin alpha V beta 8 is expressed by cells of the immune system, most prominently on CD4+ T cells and on dendritic cells (8). Unlike other alpha V integrins, alpha V beta 8 does not interact with the cytoskeleton or activate cytoplasmic signaling pathways (3, 9). Instead, it binds ligands containing an arginine-glycine-aspartic acid (RGD) motif, including Vitronectin, Fibrin and the latency associated peptide (LAP) (10, 11). High affinity binding of alpha V beta 8 to LAP triggers the MT1-MMP induced proteolytic cleavage of LAP and the release of active TGF-beta (12). Active TGF-beta regulates cell growth and nearby vascularization (5, 12, 13). Furthermore, Integrin alpha V beta 8-mediated TGF-beta activation in specialized dendritic cells of the intestine is crucial for maintaining immune homeostasis in the gut (14). Deletion of either alpha V or beta 8 reveals that alpha V beta 8 is required for vascular morphogenesis in the embryonic brain (15-16). The 958 amino acid mouse alpha V extracellular domain (ECD) shares 92% and 88% aa sequence identity with human and rat alpha V, respectively, while the 637 aa mouse beta 8 ECD shares 87% and 96% aa sequence identity with human and rat orthologs, respectively.
- Luo, BH. et al. (2007) Annu.Rev.Imm.25:619.
- Milner, R. et al. (1997) Glia 21:350.
- Nishimura, S. et al. (1998) Brain Res. 791:271.
- Zhu, J. et al. (2002) Development 129:2891.
- Cambier, S. et al. (2000) Cancer Res. 60:7084.
- Khan, S. et al. (2011) Am. J. Pathol. 178:609.
- Araya, J. et al. (2006) Am. J. Pathol. 169:405.
- Travis, M.A. et al. (2007) Nature 449:361.
- Moyle, M. et al. (1991) J. Biol. Chem. 266:19650.
- Nishimura, S. et al. (1994) J. Biol. Chem. 269:28708.
- Chernousov, M. A. and D. J. Carey (2003) Exp. Cell Res. 291:514.
- Mu, D. et al. (2002) J. Cell Biol. 157:493.
- Araya, J. et al. (2006) Am. J. Pathol. 169:405.
- Worthington, J.J. et al. (2012) Immunobiology 217:1259.
- Cambier, S. et al. (2005) Am. J. Pathol. 166:1883.
- Proctor, J. M. et al. (2005) J. Neurosci. 25:9940.
- McCarty, J. H. et al. (2005) Development 132:165.
Citation for Recombinant Mouse Integrin alpha V beta 8 Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
The RGD-binding integrins alphavbeta6 and alphavbeta8 are receptors for mouse adenovirus-1 and -3 infection
Authors: M Bieri, R Hendrickx, M Bauer, B Yu, T Jetzer, B Dreier, PRE Mittl, J Sobek, A Plückthun, UF Greber, S Hemmi
PloS Pathogens, 2021;17(12):e1010083.
Sample Types: Whole Cells
What is the amino acid sequence of the acidic and basic tails?
Acidic and basic tails are added to the protein to help facilitate optimal activity. While we generally include sequence information on the product datasheet, the sequences of these tails are considered confidential information.
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