Recombinant Mouse Integrin alpha V beta 8 Protein, CF

R&D Systems | Catalog # 8314-AV

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Key Product Details

  • R&D Systems CHO-derived Recombinant Mouse Integrin alpha V beta 8 Protein (8314-AV)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

CHO

Accession Number

P43406 & Q0VBD0

Structure / Form

Non-covalent heterodimer

Applications

Bioactivity
View all Integrin alpha V beta 8 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived mouse Integrin alpha V beta 8 protein
Mouse Integrin aV
(Phe31-Val988)
Accession # P43406		 His-Pro GGGSGGGS Acidic Tail 6-His tag
Mouse Integrin beta 8
(Glu43-Ser679)
Accession # Q0VBD0		 His-Pro GGGSGGGS Basic Tail
N-terminus C-terminus

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Phe31 ( alpha V subunit) & Glu43 ( beta 8 subunit) 

Predicted Molecular Mass

115 kDa ( alpha V subunit), 78 kDa ( beta 8 subunit)

SDS-PAGE

95 - 170 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
Immobilized Recombinant Mouse Integrin alpha V beta 8 at 2 μg/mL can bind Recombinant Human LAP (TGF-beta 1) (Catalog # 246-LP) with an apparent Kd <0.1 nM.

Formulation, Preparation, and Storage

8314-AV
Formulation Lyophilized from a 0.2 μm filtered solution in PBS and Trehalose.
Reconstitution

Reconstitute at 500 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Integrin alpha V beta 8

Integrin  alpha V beta 8 is a type I transmembrane non-covalent heterodimer composed of a 115 kDa alpha V/CD51 subunit and an 84 kDa beta 8 subunit. The Integrin  beta 8 exclusively partners with the alpha V subunit, while alpha V forms heterodimers with beta 1, beta 3, beta 5 and beta 6 (1). Integrin  alpha V beta 8 is expressed on Schwann cells and astrocytes in the brain, vascular epithelial cells, mesangial cells in the kidney, and fibroblasts and epithelial cells in the airway (2-7). Interestingly, Integrin alpha V beta 8 is expressed by cells of the immune system, most prominently on CD4+ T cells and on dendritic cells (8). Unlike other alpha V integrins, alpha V beta 8 does not interact with the cytoskeleton or activate cytoplasmic signaling pathways (3, 9). Instead, it binds ligands containing an arginine-glycine-aspartic acid (RGD) motif, including Vitronectin, Fibrin and the latency associated peptide (LAP) (10, 11). High affinity binding of alpha V beta 8 to LAP triggers the MT1-MMP induced proteolytic cleavage of LAP and the release of active TGF-beta (12). Active TGF-beta regulates cell growth and nearby vascularization (5, 12, 13). Furthermore, Integrin alpha V beta 8-mediated TGF-beta activation in specialized dendritic cells of the intestine is crucial for maintaining immune homeostasis in the gut (14). Deletion of either alpha V or beta 8 reveals that alpha V beta 8 is required for vascular morphogenesis in the embryonic brain (15-16). The 958 amino acid mouse alpha V extracellular domain (ECD) shares 92% and 88% aa sequence identity with human and rat alpha V, respectively, while the 637 aa mouse beta 8 ECD shares 87% and 96% aa sequence identity with human and rat orthologs, respectively.

References

  1. Luo, BH. et al. (2007) Annu.Rev.Imm.25:619.
  2. Milner, R. et al. (1997) Glia 21:350.
  3. Nishimura, S. et al. (1998) Brain Res. 791:271.
  4. Zhu, J. et al. (2002) Development 129:2891.
  5. Cambier, S. et al. (2000) Cancer Res. 60:7084.
  6. Khan, S. et al. (2011) Am. J. Pathol. 178:609.
  7. Araya, J. et al. (2006) Am. J. Pathol. 169:405.
  8. Travis, M.A. et al. (2007) Nature 449:361.
  9. Moyle, M. et al. (1991) J. Biol. Chem. 266:19650.
  10. Nishimura, S. et al. (1994) J. Biol. Chem. 269:28708.
  11. Chernousov, M. A. and D. J. Carey (2003) Exp. Cell Res. 291:514.
  12. Mu, D. et al. (2002) J. Cell Biol. 157:493.
  13. Araya, J. et al. (2006) Am. J. Pathol. 169:405.
  14. Worthington, J.J. et al. (2012) Immunobiology 217:1259.
  15. Cambier, S. et al. (2005) Am. J. Pathol. 166:1883.
  16. Proctor, J. M. et al. (2005) J. Neurosci. 25:9940.
  17. McCarty, J. H. et al. (2005) Development 132:165.

Entrez Gene IDs

3685 (Human)

Gene Symbol

ITGAV

UniProt

P43406 & Q0VBD0

Additional Integrin alpha V beta 8 Products

Product Documents for Recombinant Mouse Integrin alpha V beta 8 Protein, CF

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Product Specific Notices for Recombinant Mouse Integrin alpha V beta 8 Protein, CF

For research use only

Citations for Recombinant Mouse Integrin alpha V beta 8 Protein, CF

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FAQs for Recombinant Mouse Integrin alpha V beta 8 Protein, CF

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  • Q: What is the amino acid sequence of the acidic and basic tails?

    A: Acidic and basic tails are added to the protein to help facilitate optimal activity. While we generally include sequence information on the product datasheet, the sequences of these tails are considered confidential information.

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