Recombinant Mouse Netrin-4 Protein

(3 citations)   
  • Purity
    >80%, by SDS-PAGE under reducing conditions and visualized by silver stain
  • Endotoxin Level
    <0.1 EU per 1 μg of the protein by the LAL method.
  • Activity

    Measured by its ability to bind rrUNC5H2/Fc Chimera in a functional ELISA.

    Immobilized rrUNC5H2/Fc Chimera at 5 µg/mL (100 µL/well) can bind rmNetrin-4 with a linear range of 6-400 ng/mL.

  • Source
    Mouse myeloma cell line, NS0-derived Leu19-Val628 & Ala26-Val628, both with a C-terminal 10-His tag
  • Accession #
  • N-terminal Sequence
    Analysis
    Leu19 & Ala26
  • Predicted Molecular Mass
    69 kDa
  • SDS-PAGE
    85 kDa, reducing conditions
Carrier Free
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
1132-N4
 
1132-N4/CF
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Netrin-4

Netrins/UNC-6 (netr: Sanskrit for “one who guides”) are a family of laminin-related small proteins that are involved in neurite outgrowth and axon guidance. Netrins binds to the DCC and UNC5 family of receptors to attract or repel axons. Mouse Netrin-4, also known as beta -Netrin, is synthesized as a 629 amino acid (aa) precursor that contains a 19 aa signal sequence, a 428 aalaminin-related region containing an N-terminal laminin globular domain (domain VI) followed by 3 laminin EGF-like repeats, and a 182 aa C domain rich in basic aa residues that serves as a heparin binding site. Netrin-4 has been reported to exist as both a monomer and a dimer. The dimeric form was reported to be the less active of the two. Mouse Netrin-4 shares 31%, 29% and 25% aa sequence identity with mouse Netrin-1, -3, and -G1a, respectively. It also shares 89% aa sequence identity with human Netrin-4. Netrin-4 has widespread expression, occurring in nervous tissues such as embryonic floor plate and postnatal neurons such as cerebellar granule cells and hippocampal pyramidal cells. It also is found in non-neural tissues such as adult Bowman’s capsule and medullary tubular epithelium in kidney, and embryonic pancreatic and intestinal epithelium, plus cells of the ureteric bud. Netrin-4 expression is often associated with basement membrane. Netrin-4 has been shown to initiate axon outgrowth from olfactory bulb explants (1 - 7).

  • References:
    1. McFarlane, S. (2000) Biochem. Cell. Biol. 78:563.
    2. Kennedy, T.E. (2000) Biochem. Cell Biol. 78:569.
    3. Yu, T.W. and C.I. Bargmann (2001) Nature Neurosci. 4(Suppl):1169.
    4. Stein, E. et al. (2001) Science 291:1976.
    5. Koch, M. et al. (2000) J. Cell Biol. 151:221.
    6. Yin, Y. et al. (2000) Mech. Dev. 96:115.
    7. Livesey, F.J. (1999) Cell. Mol. Life Sci. 56:62.
  • Entrez Gene IDs:
    59277 (Human); 57764 (Mouse)
  • Alternate Names:
    beta-Netrin; hepar-derived netrin-like protein; netrin 4; Netrin4; Netrin-4; NTN4; PRO3091
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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Species
Applications
Sample Type
  1. Therapeutic effects of topical netrin-4 inhibits corneal neovascularization in alkali-burn rats.
    Authors: Han, Yun, Shao, Yi, Liu, Tingting, Qu, Yang-Luo, Li, Wei, Liu, Zuguo
    PLoS ONE, 2015;10(4):e0122951.
    Species: Rat
    Sample Type: In Vivo
    Application: In Vivo
  2. Netrin-4 enhances angiogenesis and neurologic outcome after cerebral ischemia.
    Authors: Hoang S, Liauw J, Choi M
    J. Cereb. Blood Flow Metab., 2009;29(2):385-97.
    Species: Mouse
    Sample Type: In Vivo
    Application: In Vivo
  3. Netrin-1 and kidney injury. I. Netrin-1 protects against ischemia-reperfusion injury of the kidney.
    Authors: Wang W, Reeves WB, Ramesh G
    Am. J. Physiol. Renal Physiol., 2008;294(4):F739-47.
    Species: Rat
    Sample Type: In Vivo
    Application: In Vivo
Primary Antibodies
Description Application Cat# Citations Images  

His Tag Antibody

WB, Simple Western, Flow, AP, CyTOF-ready MAB050 26  
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His Tag HRP-conjugated Antibody

WB, Simple Western MAB050H 3  
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His Tag APC-conjugated Antibody

ICFlow IC050A  
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His Tag Biotinylated Antibody

WB BAM050 4  
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His Tag PE-conjugated Antibody

ICFlow IC050P 2  
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His Tag Antibody

WB, Flow, AP MAB050R  
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His Tag Alexa Fluor® 488-conjugated Antibody

ICFlow IC050G  
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His Tag PerCP-conjugated Antibody

ICFlow IC050C  
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His Tag Alexa Fluor® 700-conjugated Antibody

ICFlow IC050N  
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Protein Purification Kits
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Histidine-tagged Protein Purification Resin

IP999 1
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