Recombinant Mouse Reelin Protein

Carrier Free

Catalog # Availability Size / Price Qty
3820-MR-025/CF

With Carrier

Catalog # Availability Size / Price Qty
3820-MR-025

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R&D Systems Recombinant Proteins and Enzymes
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Product Details
Citations (7)
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Recombinant Mouse Reelin Protein Summary

Product Specifications

Purity
>85%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to bind biotinylated rhApoE R2 in a functional ELISA.
Source
Mouse myeloma cell line, NS0-derived mouse Reelin protein
Leu1221-Ile2661, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Analysis
Leu1221
Predicted Molecular Mass
162.1 kDa
SDS-PAGE
145-160 kDa, reducing conditions

Product Datasheets

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3820-MR (with carrier)

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3820-MR/CF (carrier free)

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

3820-MR

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

3820-MR/CF

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Background: Reelin

Reelin is a secreted modular glycoprotein that exhibits serine protease activity and is crucial for brain development and function (1-3). It is composed of an N-terminal Reelin domain, eight EGF-like Reelin repeats (RR), and a highly basic C-terminal region (4-6). The N-terminal fragment is suggested to mediate dimerization/oligomerization and receptor recognition, the midpiece receptor binding, and the C-terminal fragment receptor signaling and recognition (1, 5, 7-9). Mouse Reelin is synthesized as a 3461amino acid (aa) precursor protein with a molecular weight of approximately 388 kDa (4). During processing, it can be cleaved between RR2 and RR3 or between RR6 and RR7, producing a 180 kDa and a 330 kDa peptide, respectively (1, 6, 10). Within shared regions in the central fragment, mouse Reelin shares 95% and 97% aa sequence identity with human and rat Reelin, respectively.

Reelin is secreted by Cajal-Retzius cells in the embryo (1, 4, 11). In the adult, it is expressed in the subventricular zone, rostral migratory stream, olfactory bulb, and in the CA1, CA3, and dentate gyrus regions of the hippocampus, as well as in cerebellar granule cells, pyramidal cells of the entorhinal cortex, GABA interneurons, and glial cells (1, 6, 12, 13). Reelin utilizes the receptors VLDLR and ApoE R2, which have been suggested to have divergent roles in Reelin-mediated neuronal migration (1, 2, 6, 12). It has also been shown to interact with Integrin alpha 3 beta 1 and APP (1, 6, 14, 15). During cortical plate development, Reelin controls cell-cell interactions critical for proper neuronal migration and positioning (1, 2, 4, 5, 11, 12, 16). In the adult, it plays a role in dendrite growth and maturation, and synapse formation (2, 6, 15). Additionally, Reelin has been shown to modulate synaptic transmission and plasticity by regulating the subunit composition and conductivity of NMDA receptors (2, 6, 17). Mutation of the human RELN gene results in lissencephaly with cerebellar hypoplasia (11, 18). In addition, abnormal Reelin expression in human brain has been associated with a variety of cognitive pathological conditions including autism, schizophrenia, bipolar disorder, major depression, and Alzheimer’s disease (1, 6, 11, 13, 19, 20). Reelin deficiency found in Reeler mutant mice causes ataxia, tremors, and impaired motor coordination (4, 16). Peripherally, Reelin is important in the development of neuromuscular junctions. But instead of utilizing the locally expressed ApoE R2 and VLDLR, this function requires the serine protease activity of Reelin (3, 21).

References
  1. Fatemi, S.H. (2005) Mol. Psychiatry 10:251.
  2. Förster, E. et al. (2010) Eur. J. Neurosci. 31:1511.
  3. Quattrocchi, C.C. et al. (2002) J. Biol. Chem. 277:303.
  4. D’Arcangelo, G. et al. (1995) Nature 374:719.
  5. Jossin, Y. et al. (2004) J. Neurosci. 24:514.
  6. Folsom, T.D. and S.H. Fatemi (2013) Neuropharmacology 68:122.
  7. Utsunomiya-Tate, N. et al. (2000) Proc. Natl. Acad. Sci. USA 97:9729.
  8. Nakano, Y. et al. (2007) J. Biol. Chem. 282:20544.
  9. Hibi, T. et al. (2009) Neurosci. Res. 63:251.
  10. Lambert de Rouvroit, C. et al. (1999) Exp. Neurol. 156:214.
  11. Meyer, G. (2010) J. Anat. 217:334.
  12. D’Arcangelo, G. et al. (1999) Neuron 24:471.
  13. Senkov, O. et al. (2014) Prog. Brain Res. 214:53.
  14. Dulabon, L. et al. (2000) Neuron 27:33.
  15. Hoe, H.S. et al. (2009) J. Neurosci. 29:7459.
  16. Hirotsune, S. et al. (1995) Nat. Genet. 10:77.
  17. Levy, A.D. et al. (2014) Front. Neuroanat. 8:116.
  18. Barros, C.S. et al. (2011) Cold Spring Harb. Perspect. Biol. 3:a005108.
  19. Botella-López, A. et al. (2006) Proc. Natl. Acad. Sci. USA 103:5573.
  20. Lubbers, B.R. et al. (2014) Prog. Brain Res. 214:263.
  21. Quattrocchi, C.C. et al. (2003) Science 301:649.
Entrez Gene IDs
5649 (Human); 19699 (Mouse); 24718 (Rat)
Alternate Names
EC 3.4.21; EC 3.4.21.-; Reeler; Reelin; RELN; RL; RLPRO1598

Citations for Recombinant Mouse Reelin Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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  1. Lymphatics act as a signaling hub to regulate intestinal stem cell activity
    Authors: RE Niec, T Chu, M Schernthan, S Gur-Cohen, L Hidalgo, HA Pasolli, KA Luckett, Z Wang, SR Bhalla, F Cambuli, RP Kataru, K Ganesh, BJ Mehrara, D Pe'er, E Fuchs
    Cell Stem Cell, 2022-06-20;29(7):1067-1082.e18.
    Species: Mouse
    Sample Types: Organoid
    Applications: Bioassay
  2. Dynamics, nanomechanics and signal transduction in reelin repeats
    Authors: K Mikulska-R, J Strzelecki, W Nowak
    Sci Rep, 2019-12-12;9(1):18974.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  3. Reelin Can Modulate Migration of Olfactory Ensheathing Cells and Gonadotropin Releasing Hormone Neurons via the Canonical Pathway
    Authors: L Dairaghi, E Flannery, P Giacobini, A Saglam, H Saadi, S Constantin, F Casoni, BW Howell, S Wray
    Front Cell Neurosci, 2018-08-03;12(0):228.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  4. Hypervulnerability of the adolescent prefrontal cortex to nutritional stress via reelin deficiency
    Mol. Psychiatry, 2016-11-15;0(0):.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  5. Reciprocal regulation of very low density lipoprotein receptors (VLDLRs) in neurons by brain-derived neurotrophic factor (BDNF) and Reelin: involvement of the E3 ligase Mylip/Idol.
    Authors: Do H, Bruelle C, Tselykh T, Jalonen P, Korhonen L, Lindholm D
    J Biol Chem, 2013-08-29;288(41):29613-20.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  6. Smooth muscle-endothelial cell communication activates Reelin signaling and regulates lymphatic vessel formation.
    J. Cell Biol., 2012-06-04;197(6):837-49.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  7. miR-200c regulates FGFR-dependent epithelial proliferation via Vldlr during submandibular gland branching morphogenesis.
    Authors: Rebustini IT, Hayashi T, Reynolds AD
    Development, 2011-11-24;139(1):191-202.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay

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Reviews for Recombinant Mouse Reelin Protein

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Recombinant Mouse Reelin Protein
By Anonymous on 04/09/2019
Application: Cell migration/motility