Recombinant Mouse Wnt-2b Protein

R&D Systems | Catalog # 3900-WN

R&D Systems
Discontinued Product
3900-WN has been discontinued. View all Wnt-2b products.

Key Product Details

Source

CHO

Accession Number

AAC25397

Applications

Bioactivity
View all Wnt-2b Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived mouse Wnt-2b protein
Ser57-Thr389

Purity

>95%, by SDS-PAGE with silver staining, under reducing conditions.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ser57 & Asp71

Predicted Molecular Mass

38 kDa

SDS-PAGE

41-60 kDa, reducing conditions

Activity

Measured by its ability to inhibit cell proliferation/survival assay using C3H10T1/2 mouse embryonic fibroblast cells.
The ED50 for this effect is 1-5 µg/ml.

Formulation, Preparation, and Storage

3900-WN
Formulation Lyophilized from a 0.2 μm filtered solution in PBS, EDTA and CHAPS with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Wnt-2b

Mouse Wnt-2b (also known as Wnt-13) is a member of the large and highly conserved Wnt family of signaling molecules that have roles in pattern formation, cell fate decision, axon guidance, and tumor formation (1). Mouse Wnt-2b is 389 amino acids (aa) in length and includes a signal peptide (2). Mature mouse Wnt-2b has a predicted molecular mass of 38 kDa and shares 96% aa sequence identity with the human protein. Wnt-2b initiates B-catenin-mediated canonical signaling and is implicated in the development of the lung, kidney, liver, and eye, as well as being present in ovarian surface epithelial cells (3-8). Pathological expression of Wnt-2b in tumors is correlated with increased cell proliferation and decreased survival (9).

References

  1. Anastas, J.N. and R.T. Moon (2013) Nat. Rev. Cancer 13:11.
  2. Zakin, L.D. et al. (1998) Mech. Dev. 73:107.
  3. Goss, A.M. et al. (2009) Dev. Cell 17:290.
  4. Ricken, A. et al. (2002) Endocrinology 143:2741.
  5. Lin, Y. et al. (2001) Dev. Dyn. 222:26.
  6. Jasoni, C. et al. (1999) Dev. Dyn. 215:215.
  7. Ober, E.A. et al. (2006) Nature 442:688.
  8. Kubo, F. et al. (2005) Development 132:2759.
  9. Kobayashi, M. et al. (2012) Exp. Ther. Med. 3:952.

Long Name

Wingless-type MMTV Integration Site Family Member 2b

Alternate Names

Wnt-13, Wnt2b, XWNT2

Entrez Gene IDs

7482 (Human); 22414 (Mouse); 116466 (Rat)

Gene Symbol

WNT2B

UniProt

AAC25397

Additional Wnt-2b Products

Product Documents for Recombinant Mouse Wnt-2b Protein

Certificate of Analysis

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Product Specific Notices for Recombinant Mouse Wnt-2b Protein

For research use only

Related Research Areas

Wnt Ligands (Wnts)

Citations for Recombinant Mouse Wnt-2b Protein

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