Recombinant Mouse Wnt-8a Protein, CF

  
  • Purity
    >95%, by SDS-PAGE with silver staining.
  • Endotoxin Level
    <0.10 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured by its ability to induce alkaline phosphatase production by C3H10T1/2 mouse embryonic fibroblast cells. The ED50 for this effect is 0.5-2.5 μg/mL.
  • Source
    Chinese Hamster Ovary cell line, CHO-derived Ala20-Trp354
  • Accession #
  • N-terminal Sequence
    Analysis
    Ala20 & Trp23
  • Predicted Molecular Mass
    37 kDa
  • SDS-PAGE
    40-62 kDa, reducing conditions
Carrier Free
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
8419-WN/CF
 
8419-WN
Formulation Lyophilized from a 0.2 μm filtered solution in PBS, NaCl, EDTA and CHAPS.
Formulation Lyophilized from a 0.2 μm filtered solution in PBS, NaCl, EDTA and CHAPS with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in PBS.
Reconstitution Reconstitute at 100 μg/mL in PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Data Images
Wnt-8a Induces the Production of Alkaline Phosphatase.
Recombinant Mouse Wnt-8a stimulates alkaline phosphatase production by C3H10T1/2 mouse embryonic fibroblast cells. The ED50 for this effect is 0.5-2.5 μg/mL.
1 μg/lane ofRecombinant Mouse Wnt-8a (Catalog # 8419-WN/CF)was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditionsand visualized by silver staining, showing bands at 42.3 and 38.9 kDa, respectively.
Background: Wnt-8a

Wnt-8a is a member of the large and highly conserved Wnt family of signaling molecules that have roles in pattern formation, cell fate decision, axon guidance, and tumor formation (1). Mouse Wnt-8a consists of a 19 amino acid (aa) signal peptide that is cleaved to release the mature 335 aa secreted protein (2). Mature mouse Wnt-8a shares 82% and 91% sequence identity with human and rat mature Wnt-8a, respectively. Similar to other canonical Wnts, Wnt-8a binds to the Frizzled family of receptors to intiate beta-catenin signaling. Wnt-8a is expressed during early embryogenesis and is involved in mesoderm patterning and posteriorization of the neuroectoderm (3-6). Wnt-8a signaling promotes optic lens development as well as otic placode formation during inner ear development (7, 8). Expression of Wnt-8a is observed in heart progenitor cells where it positively regulates cardiac myogenesis (9, 10).

  • References:
    1. Anastas, J.N. and R.T. Moon (2013) Nat. Rev. Cancer 13:11.
    2. Bouillet, P. et al. (1996) Mech. Dev. 58:141.
    3. Rhinn, M. et al. (2005) Development 132:1261.
    4. Lekven, A.C. et al. (2001) Dev. Cell. 1:103.
    5. Erter, C.E. et al. (2001) Development 128:3571.
    6. Stump, R.J. et al. (2003) Dev. Biol. 259:48.
    7. Vendrell, V. et al. (2013) Mech. Dev. 130:160.
    8. Chong, C.C. et al. (2009) Exp. Eye Res. 88:307.
    9. Nakamura, T. et al. (2003) Proc. Natl. Acad. Sci. USA 100:5834.
    10. Kwon, C. et al. (2007) Proc. Natl. Acad. Sci. USA 104:10894.
  • Long Name:
    Wingless-type MMTV Integration Site Family, Member 8a
  • Entrez Gene IDs:
    7478 (Human); 20890 (Mouse)
  • Alternate Names:
    protein Wnt-8a; Protein Wnt-8d; wingless-type MMTV integration site family, member 8A; Wnt8a; Wnt-8a; WNT8DWNT8d
Related Research Areas

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