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Recombinant Plant Ubiquitin Protein, CF

Catalog #: U-100At
5 Citations Datasheet / COA / SDS

Discontinued Product

U-100At has been discontinued.
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Citations (5)
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Recombinant Plant Ubiquitin Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain
Activity
Recombinant Plant Ubiquitin can be conjugated to substrate proteins via the subsequent actions of a Ubiquitin-activating (E1) enzyme, a Ubiquitin-conjugating (E2) enzyme, and a Ubiquitin ligase (E3). Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Plant Ubiquitin concentration of 0.5-1 mM.
Source
E. coli-derived plant Ubiquitin protein
Accession #
NP_001190681
Predicted Molecular Mass
8.6 kDa

Product Datasheets

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U-100AT

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

U-100At

Formulation Lyophilized from deionized water.
Reconstitution Reconstitute at 10 mg/mL in an aqueous solution. 
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Ubiquitin

Ubiquitin is a 76 amino acid (aa) protein that is ubiquitously expressed in all eukaryotic organisms. Ubiquitin is highly conserved with 96% aa sequence identity shared between human and yeast Ubiquitin, and 100% aa sequence identity shared between human and mouse Ubiquitin (1). In mammals, four Ubiquitin genes encode for two Ubiquitin-ribosomal fusion proteins and two poly-Ubiquitin proteins. Cleavage of the Ubiquitin precursors by deubiquitinating enzymes gives rise to identical Ubiquitin monomers each with a predicted molecular weight of 8.6 kDa. Conjugation of Ubiquitin to target proteins involves the formation of an isopeptide bond between the C-terminal glycine residue of Ubiquitin and a lysine residue in the target protein. This process of conjugation, referred to as ubiquitination or ubiquitylation, is a multi-step process that requires three enzymes: a Ubiquitin-activating (E1) enzyme, a Ubiquitin-conjugating (E2) enzyme, and a Ubiquitin ligase (E3). Ubiquitination is classically recognized as a mechanism to target proteins for degradation and as a result, Ubiquitin was originally named ATP-dependent Proteolysis Factor 1 (APF-1) (2,3). In addition to protein degradation, ubiquitination has been shown to mediate a variety of biological processes such as signal transduction, endocytosis, and post-endocytic sorting (4-7).

Highly purified Ubiquitin free of glycine and buffer salts which can interfere with chemical and in vitro reactions. This protein sequence is for A.thaliana (P59263) and is exactly the same for barley, oat, soya bean and other plants.

References
  1. Sharp, P.M. & W.-H. Li. (1987) Trends Ecol. Evol. 2:328.
  2. Ciechanover, A. et al. (1980 ) Proc. Natl. Acad. Sci. USA 77:1365.
  3. Hershko, A. et al. (1980) Proc. Natl. Acad. Sci. USA 77:1783.
  4. Greene, W. et al. (2012) PLoS Pathog. 8:e1002703.
  5. Tong, X. et al. (2012) J. Biol. Chem. 287:25280.
  6. Wei, W. et al. (2004) Nature 428:194.
  7. Wertz, I.E. et al. (2004) Nature 430:694.
Entrez Gene IDs
7314 (Human); 298693 (Rat)
Alternate Names
RPS27A; UBA52; UBB ubiquitin B; UBB; UBC; Ubiquitin

Citations for Recombinant Plant Ubiquitin Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. An m6A methyltransferase confers host resistance by degrading viral proteins through ubiquitination
    Authors: Guo, J;Zhang, T;Xie, H;Hu, H;Shi, C;Zhao, Y;Yin, J;Xu, G;Wu, Z;Wang, P;Liu, J;Liu, P;Zhong, K;Chen, F;Chen, J;Yang, J;
    Nature communications
    Species: N/A
    Sample Types: Recombinant Protein
    Applications: Bioassay
  2. The rice peroxisomal receptor PEX5 negatively regulates resistance to rice blast fungus Magnaporthe oryzae
    Authors: You, X;Zhu, S;Sheng, H;Liu, Z;Wang, D;Wang, M;Xu, X;He, F;Fang, H;Zhang, F;Wang, D;Hao, Z;Wang, R;Xiao, Y;Wan, J;Wang, GL;Ning, Y;
    Cell reports
    Species: N/A
    Sample Types: Recombinant Protein
    Applications: Bioassay
  3. An ORFeome of rice E3 ubiquitin ligases for global analysis of the ubiquitination interactome
    Authors: R Wang, X You, C Zhang, H Fang, M Wang, F Zhang, H Kang, X Xu, Z Liu, J Wang, Q Zhao, X Wang, Z Hao, F He, H Tao, D Wang, J Wang, L Fang, M Qin, T Zhao, P Zhang, H Xing, Y Xiao, W Liu, Q Xie, GL Wang, Y Ning
    Genome Biology, 2022-07-11;23(1):154.
    Species: Plant - Oryza sativa (Rice)
    Sample Types: Recombinant Proteins
    Applications: Bioassay
  4. The E3 ligase MREL57 modulates microtubule stability and stomatal closure in response to ABA
    Authors: L Dou, K He, J Peng, X Wang, T Mao
    Nature Communications, 2021-04-12;12(1):2181.
    Species: Human
    Sample Types: Peptide
    Applications: Bioassay
  5. The MtDMI2-MtPUB2 negative feedback loop plays a role in nodulation homeostasis
    Authors: J Liu, J Deng, F Zhu, Y Li, Z Lu, P Qin, T Wang, J Dong
    Plant Physiol., 2018-02-13;0(0):.
    Species: Plant - Medicago truncatula
    Sample Types: Recombinant Protein
    Applications: Bioassay

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