RFX5 Antibody

Novus Biologicals | Catalog # NBP2-26106

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Predicted:

Bovine (100%), Canine (100%), Porcine (100%), Rabbit (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Western Blot, Peptide ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all RFX5 Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-TGDKSSEPSTLSNE corresponding to internal region according to NP_000440.1.

Specificity

Reported variants represent identical protein: NP_000440.1, NP_001020774.1

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for RFX5 Antibody

Western Blot: RFX5 Antibody [NBP2-26106]

Western Blot: RFX5 Antibody [NBP2-26106]

Western Blot: RFX5 Antibody [NBP2-26106] - Staining of NIH3T3 nuclear lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Western Blot: RFX5 Antibody [NBP2-26106]

Western Blot: RFX5 Antibody [NBP2-26106]

Western Blot: RFX5 Antibody [NBP2-26106] - Staining of Daudi lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Applications for RFX5 Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:1000

Western Blot

1 - 3 ug/ml
Application Notes
WB: Approx. 70 kDa band observed in lysates of Daudi and in NIH3T3 nuclear lysates (calculated MW of 65.3 kDa band according to NP_000440.1).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: RFX5

RFX5 is the regulatory gene responsible for human MHC class II deficiency. Patients with this disease exhibit a characteristic defect in the binding of a nuclear factor, RFX, to the X box motif of MHC class II promoters. RFX5 is the 75 kDa subunit of the RFX complex which is absolutely essential and highly specific for regulating MHC class II gene expression and control of the immune response. RFX5 is a protein consisting of 616 amino acids.

Alternate Names

DNA-binding protein RFX5, Regulatory factor X 5, regulatory factor X, 5 (influences HLA class II expression)

Entrez Gene IDs

5993 (Human); 53970 (Mouse); 310659 (Rat)

Gene Symbol

RFX5

Additional RFX5 Products

Product Documents for RFX5 Antibody

Certificate of Analysis

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Product Specific Notices for RFX5 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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