RhoC Antibody (2E12) - Azide and BSA Free

Novus Biologicals | Catalog # H00000389-M01

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, ELISA, Sandwich ELISA

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # 2E12

Format

Azide and BSA Free
View all RhoC Primary Antibodies »

Product Specifications

Immunogen

RHOC (AAH07245, 1 a.a. ~ 193 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MAAIRKKLVIVGDGACGKTCLLIVFSKDQFPEVYVPTVFENYIADIEVDGKQVELALWDTAGQEDYDRLRPLSYPDTDVILMCFSIDSPDSLENIPEKWTPEVKHFCPNVPIILVGNKKDLRQDEHTRRELAKMKQEPVRSEEGRDMANRISAFGYLECSAKTKEGVREVFEMATRAGLQVRKNKRRRGCPIL

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Specificity

RHOC (2E12)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Theoretical MW

48 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for RhoC Antibody (2E12) - Azide and BSA Free

Western Blot: RhoC Antibody (2E12) [H00000389-M01]

Western Blot: RhoC Antibody (2E12) [H00000389-M01]

Western Blot: RhoC Antibody (2E12) [H00000389-M01] - Analysis of RHOC expression in transfected 293T cell line by RHOC monoclonal antibody (M01), clone 2E12.Lane 1: RHOC transfected lysate(22 KDa).Lane 2: Non-transfected lysate.
Western Blot: RhoC Antibody (2E12) [H00000389-M01]

Western Blot: RhoC Antibody (2E12) [H00000389-M01]

Western Blot: RhoC Antibody (2E12) [H00000389-M01] - RHOC monoclonal antibody (M01), clone 2E12. Analysis of RHOC expression in PC-12.
Western Blot: RhoC Antibody (2E12) [H00000389-M01]

Western Blot: RhoC Antibody (2E12) [H00000389-M01]

Western Blot: RhoC Antibody (2E12) [H00000389-M01] - RHOC monoclonal antibody (M01), clone 2E12 Analysis of RHOC expression in Jurkat.
Western Blot: RhoC Antibody (2E12) [H00000389-M01]

Western Blot: RhoC Antibody (2E12) [H00000389-M01]

Western Blot: RhoC Antibody (2E12) [H00000389-M01] - RHOC monoclonal antibody (M01), clone 2E12. Analysis of RHOC expression in NIH/3T3.
Sandwich ELISA: RhoC Antibody (2E12) [H00000389-M01] - Detection limit for recombinant GST tagged RHOC is approximately 3ng/ml as a capture antibody.

Applications for RhoC Antibody (2E12) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: RhoC

This gene encodes a member of the Rho family of small GTPases, which cycle between inactive GDP-bound and active GTP-bound states and function as molecular switches in signal transduction cascades. Rho proteins promote reorganization of the actin cytoskeleton and regulate cell shape, attachment, and motility. The protein encoded by this gene is prenylated at its C-terminus, and localizes to the cytoplasm and plasma membrane. It is thought to be important in cell locomotion. Overexpression of this gene is associated with tumor cell proliferation and metastasis. Multiple alternatively spliced variants, encoding the same protein, have been identified.

Alternate Names

ARH9ARHCRho cDNA clone 9, H9, MGC1448, MGC61427, oncogene RHO H9, ras homolog gene family, member C, RAS-related homolog 9, RhoC, rhoC GTPase, RHOH9, rho-related GTP-binding protein RhoC, small GTP binding protein RhoC

Entrez Gene IDs

389 (Human); 11853 (Mouse); 295342 (Rat)

Gene Symbol

RHOC

OMIM

165380 (Human)

UniProt

P08134 (Human), Q62159 (Mouse)

Additional RhoC Products

Product Documents for RhoC Antibody (2E12) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for RhoC Antibody (2E12) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for RhoC Antibody (2E12) - Azide and BSA Free

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