RNase H1 Antibody (5D10) - Azide and BSA Free

Novus Biologicals | Catalog # H00246243-M01

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Rat

Cited:

Human

Applications

Validated:

Western Blot, ELISA

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 5D10

Format

Azide and BSA Free
View all RNase H1 Primary Antibodies »

Product Specifications

Immunogen

RNASEH1 (NP_002927, 189 a.a. ~ 286 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. AACKAIEQAKTQNINKLVLYTDSMFTINGITNWVQGWKKNGWKTSAGKEVINKEDFVALERLTQGMDIQWMHVPGHSGFIGNEEADRLAREGAKQSED

Specificity

RNASEH1 - ribonuclease H1

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for RNase H1 Antibody (5D10) - Azide and BSA Free

Western Blot: RNase H1 Antibody (5D10) [H00246243-M01]

Western Blot: RNase H1 Antibody (5D10) [H00246243-M01]

Western Blot: RNase H1 Antibody (5D10) [H00246243-M01] - RNASEH1 monoclonal antibody (M01), clone 5D10. Analysis of RNASEH1 expression in PC-12.
Western Blot: RNase H1 Antibody (5D10) [H00246243-M01]

Western Blot: RNase H1 Antibody (5D10) [H00246243-M01]

Western Blot: RNase H1 Antibody (5D10) [H00246243-M01] - RNASEH1 monoclonal antibody (M01), clone 5D10 Analysis of RNASEH1 expression in HeLa.
Western Blot: RNase H1 Antibody (5D10) [H00246243-M01]

Western Blot: RNase H1 Antibody (5D10) [H00246243-M01]

Western Blot: RNase H1 Antibody (5D10) [H00246243-M01] - Analysis of RNASEH1 expression in transfected 293T cell line by RNASEH1 monoclonal antibody (M01), clone 5D10.Lane 1: RNASEH1 transfected lysate(32.1 KDa).Lane 2: Non-transfected lysate.
ELISA: RNase H1 Antibody (5D10) [H00246243-M01]

ELISA: RNase H1 Antibody (5D10) [H00246243-M01]

ELISA: RNase H1 Antibody (5D10) [H00246243-M01] - Detection limit for recombinant GST tagged RNASEH1 is approximately 0.03ng/ml as a capture antibody.

Applications for RNase H1 Antibody (5D10) - Azide and BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Western Blot

1:500
Application Notes
Antibody reactive against cell lysate and recombinant protein for western blot. It has also been used for ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: RNase H1

Endonuclease that specifically degrades the RNA of RNA-DNA hybrids

Alternate Names

EC 3.1.26.4, H1RNA, Ribonuclease H type II, ribonuclease H1, RNase H1, RNH1

Entrez Gene IDs

246243 (Human)

Gene Symbol

RNASEH1

OMIM

604123 (Human)

UniProt

NP_002927 (Human)

Additional RNase H1 Products

Product Documents for RNase H1 Antibody (5D10) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for RNase H1 Antibody (5D10) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for RNase H1 Antibody (5D10) - Azide and BSA Free

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