RNASEH2A Antibody (3G5-F5) - Azide and BSA Free

Novus Biologicals | Catalog # H00010535-M01

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Sandwich ELISA, Knockdown Validated

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 3G5-F5

Format

Azide and BSA Free
View all RNASEH2A Primary Antibodies »

Product Specifications

Immunogen

RNASEH2A (AAH11748, 1 a.a. ~ 299 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MDLSELERDNTGRCRLSSPVPAVCRKEPCVLGVDEAGRGPVLGPMVYAICYCPLPRLADLEALKVADSKTLLESERERLFAKMEDTDFVGWALDVLSPNLISTSMLGRVKYNLNSLSHDTATGLIQYALDQGVNVTQVFVDTVGMPETYQARLQQSFPGIEVTVKAKADALYPVVSAASICAKVARDQAVKKWQFVEKLQDLDTDYGSGYPNDPKTKAWLKEHVEPVFGFPQFVRFSWRTAQTILEKEAEDVIWEDSASENQEGLRKITSYFLNEGSQARPRSSHRYFLERGLESATSL

Reactivity Notes

Human. Other species not tested.

Localization

Nuclear

Specificity

RNASEH2A - ribonuclease H2, large subunit

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for RNASEH2A Antibody (3G5-F5) - Azide and BSA Free

Western Blot: RNASEH2A Antibody (3G5-F5) [H00010535-M01]

Western Blot: RNASEH2A Antibody (3G5-F5) [H00010535-M01]

Western Blot: RNASEH2A Antibody (3G5-F5) [H00010535-M01] - RNASEH2A monoclonal antibody (M01), clone 3G5-F5 Analysis of RNASEH2A expression in Jurkat.
Western Blot: RNASEH2A Antibody (3G5-F5) [H00010535-M01]

Western Blot: RNASEH2A Antibody (3G5-F5) [H00010535-M01]

Western Blot: RNASEH2A Antibody (3G5-F5) [H00010535-M01] - Analysis of RNASEH2A over-expressed 293 cell line, cotransfected with RNASEH2A Validated Chimera RNAi ( Cat # H00010535-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with RNASEH2A monoclonal antibody (M01), clone 3G5-F5 (Cat # H00010535-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Western Blot: RNASEH2A Antibody (3G5-F5) [H00010535-M01]

Western Blot: RNASEH2A Antibody (3G5-F5) [H00010535-M01]

Western Blot: RNASEH2A Antibody (3G5-F5) [H00010535-M01] - Analysis of RNASEH2A expression in transfected 293T cell line by RNASEH2A monoclonal antibody (M01), clone 3G5-F5.Lane 1: RNASEH2A transfected lysate(33.4 KDa).Lane 2: Non-transfected lysate.
Sandwich ELISA: RNASEH2A Antibody (3G5-F5) [H00010535-M01] - Detection limit for recombinant GST tagged RNASEH2A is 1 ng/ml as a capture antibody.

Applications for RNASEH2A Antibody (3G5-F5) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactive against cell lysate and recombinant protein for western blot. It has also been used for ELISA and RNAi Validation.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: RNASEH2A

Of the multiple RNases H in mammals, RNase HI is the major enzyme and shows increased activity during DNA replication. It shows more homology to the RNase HII of Escherichia coli.

Alternate Names

AGS4RNase H2 subunit A, Aicardi-Goutieres syndrome 4, Aicardi-Goutieres syndrome 4 protein, JUNB, ribonuclease H2 subunit A, ribonuclease H2, large subunit, ribonuclease H2, subunit A, Ribonuclease HI large subunit, Ribonuclease HI subunit A, ribonuclease HI, large subunit, RNase H(35), RNASEHIEC 3.1.26.4, RNHIARNase HI large subunit, RNHL

Entrez Gene IDs

10535 (Human)

Gene Symbol

RNASEH2A

OMIM

606034 (Human)

UniProt

AAH11748 (Human)

Additional RNASEH2A Products

Product Documents for RNASEH2A Antibody (3G5-F5) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for RNASEH2A Antibody (3G5-F5) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for RNASEH2A Antibody (3G5-F5) - Azide and BSA Free

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