rS. Solfataricus Dpo-4 with Buffer and Cations
rS. Solfataricus Dpo-4 with Buffer and Cations Summary
Product Description
Dpo4 is a thermophilic Y-family DNA polymerase capable of performing translesion synthesis and allows for coupling to PCR for amplification of damaged DNA. Dpo4 has a molecular weight of 42 kDa and is 95% pure by SDS-PAGE. Translesion synthesis is enhanced by the presence of Mn2+ in the reaction. One unit of enzyme incorporates 1 nmole of dNTP onto the 3’ end of annealed primer in 30 minutes at 60°C.
Assay Conditions and Analysis
1X REC Buffer 15 [10 mM HEPES-NaOH (pH 7.4), 1 mM DTT, 50 mM NaCl, 100 μg/ml BSA, 0.1% Triton X-100], 5 mM MgCl2, 4 pmoles of labeled annealed primer, and serial dilutions of enzyme in a 20 μl reaction volume are incubated for 30 minutes at 60°C. Reaction products are resolved by 20% denaturing polyacrylamide gel electrophoresis, and percent primer extended.
Kit Contents
• 10X REC™ Buffer 15
• 100 mM MgCl2
• 50 mM MnCl2
• S. solfataricus Dpo4
Specifications
Limitations
For research use only. Not for diagnositic use.
Product Datasheets
FAQs
No product specific FAQs exist for this product, however you may
View all FAQsReviews for rS. Solfataricus Dpo-4 with Buffer and Cations
There are currently no reviews for this product. Be the first to review rS. Solfataricus Dpo-4 with Buffer and Cations and earn rewards!
Have you used rS. Solfataricus Dpo-4 with Buffer and Cations?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
