rS. Solfataricus Dpo-4 with Buffer and Cations

4150-050-EB Datasheet / COA / SDS

Discontinued Product

4150-050-EB has been discontinued.
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rS. Solfataricus Dpo-4 with Buffer and Cations Summary

Kit containing rS.Sofataricus Dpo-4, buffers, and cations.

Product Description

Dpo4 is a thermophilic Y-family DNA polymerase capable of performing translesion synthesis and allows for coupling to PCR for amplification of damaged DNA. Dpo4 has a molecular weight of 42 kDa and is 95% pure by SDS-PAGE. Translesion synthesis is enhanced by the presence of Mn2+ in the reaction. One unit of enzyme incorporates 1 nmole of dNTP onto the 3’ end of annealed primer in 30 minutes at 60°C.

Assay Conditions and Analysis

1X REC Buffer 15 [10 mM HEPES-NaOH (pH 7.4), 1 mM DTT, 50 mM NaCl, 100 μg/ml BSA, 0.1% Triton X-100], 5 mM MgCl2, 4 pmoles of labeled annealed primer, and serial dilutions of enzyme in a 20 μl reaction volume are incubated for 30 minutes at 60°C. Reaction products are resolved by 20% denaturing polyacrylamide gel electrophoresis, and percent primer extended.

Kit Contents

• 10X REC™ Buffer 15
• 100 mM MgCl2
• 50 mM MnCl2
• S. solfataricus Dpo4

Specifications

Shipping Conditions
The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended on the product label.
Storage
Store the unopened product at -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.
Species
N/A

Limitations

For research use only. Not for diagnositic use.

Product Datasheets

Reagents Provided

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