Septin-7 Antibody

Name: Septin-7 Antibody
Brand: Novus Biologicals
SKU: NBP1-06077
Price: 399 USD
Availability: InStock

Novus Biologicals | Catalog # NBP1-06077

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bovine (100%), Canine (100%), Equine (100%), Mouse (100%), Porcine (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Western Blot, Peptide ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

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Product Specifications

Immunogen

Peptide with sequence C-DNNKNKGQLTKSP corresponding to internal region according to NP_001011553.2, NP_001229885.1.

Epitope

C-DNNKNKGQLTKSP

Specificity

This antibody is expected to recognize all three reported isoforms (NP_001779.3; NP_001011553.2; NP_001229885.1).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Septin-7 Antibody

Western Blot: Septin-7 Antibody [NBP1-06077]

Western Blot: Septin-7 Antibody [NBP1-06077] - Staining of nuclear HeLa lysate (35 ug protein in RIPA buffer). Antibody at 0.5 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.

Applications for Septin-7 Antibody

Application

Recommended Usage

Peptide ELISA

Detection limit 1:32000

Western Blot

0.3 - 1 ug/mL

Application Notes

WB: Approx. 45 kDa band observed in HeLa nuclear lysates (calculated MW of 50.7 kDa band according to NP_001779.3 and of 55.6 kDa band according to NP_001011553.2). The observed molecular weight corresponds to earlier findings with different antibodies from other sources.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Septin-7

This gene encodes a protein that is highly similar to the CDC10 protein of Saccharomyces cerevisiae. The protein also shares similarity with Diff 6 of Drosophila and with H5 of mouse. Each of these similar proteins, including the yeast CDC10, contains a GTP-binding motif. The yeast CDC10 protein is a structural component of the 10 nm filament which lies inside the cytoplasmic membrane and is essential for cytokinesis. Although the exact function of this gene has not yet been determined, its high similarity to yeast CDC10 and the high conservative nature of eukaryotic cell cycle machinery suggest a similar role to that of its yeast counterpart. Alternative splicing results in two transcript variants encoding different isoforms. [provided by RefSeq]

Alternate Names

CDC10 cell division cycle 10 homolog, CDC10 cell division cycle 10 homolog (S. cerevisiae), CDC10 protein homolog, CDC10S. cerevisiae, homolog), Nbla02942, septin 7, septin-7

Entrez Gene IDs

989 (Human); 235072 (Mouse); 64551 (Rat)

Gene Symbol

SEPTIN7

UniProt

NP_001779.3 (Human)

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Product Documents for Septin-7 Antibody

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Certificate of Analysis

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Product Specific Notices for Septin-7 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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