SH2D1A Antibody (1C9) - Azide and BSA Free

Novus Biologicals | Catalog # H00004068-M01

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Western Blot, ELISA, Flow Cytometry, Functional

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 1C9

Format

Azide and BSA Free
View all SH2D1A Primary Antibodies »

Product Specifications

Immunogen

SH2D1A (AAH20732, 1 a.a. ~ 128 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MDAVAVYHGKISRETGEKLLLATGLDGSYLLRDSESVPGVYCLCVLYHGYIYTYRVSQTETGSWSAETAPGVHKRYFRKIKNLISAFQKPDQGIVIPLQYPVEKKSSARSTQGTTGIREDPDVCLKAP

Specificity

SH2D1A - SH2 domain protein 1A, Duncan's disease (lymphoproliferative syndrome)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for SH2D1A Antibody (1C9) - Azide and BSA Free

Western Blot: SH2D1A Antibody (1C9) [H00004068-M01]

Western Blot: SH2D1A Antibody (1C9) [H00004068-M01]

Western Blot: SH2D1A Antibody (1C9) [H00004068-M01] - Analysis of SH2D1A expression in transfected 293T cell line by SH2D1A monoclonal antibody (M01), clone 1C9.Lane 1: SH2D1A transfected lysate(14.2 KDa).Lane 2: Non-transfected lysate.
Flow Cytometry: SH2D1A Antibody (1C9) [H00004068-M01]

Flow Cytometry: SH2D1A Antibody (1C9) [H00004068-M01]

Flow Cytometry: SH2D1A Antibody (1C9) [H00004068-M01] - Intracellular staining of human T cells with SH2D1A monoclonal antibody or isotype control antibody, followed by a Polyclonal Goat Anti-Mouse/RPE secondary antibody. Grey filled histogram: Isotype control, empty blue histogram: SH2D1A antibody. Flow cytometry image submitted by a verified customer review.
Western Blot: SH2D1A Antibody (1C9) [H00004068-M01]

Western Blot: SH2D1A Antibody (1C9) [H00004068-M01]

Western Blot: SH2D1A Antibody (1C9) [H00004068-M01] - SH2D1A monoclonal antibody (M01), clone 1C9 Analysis of SH2D1A expression in Jurkat.
ELISA: SH2D1A Antibody (1C9) [H00004068-M01]

ELISA: SH2D1A Antibody (1C9) [H00004068-M01]

ELISA: SH2D1A Antibody (1C9) [H00004068-M01] - Detection limit for recombinant GST tagged SH2D1A is 0.3 ng/ml as a capture antibody.

Applications for SH2D1A Antibody (1C9) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500

Reviewed Applications

Read 1 review rated 5 using H00004068-M01 in the following applications:

Flow Cytometry Panel Builder

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: SH2D1A

This gene encodes a protein that plays a major role in the bidirectional stimulation of T and B cells. This protein contains an SH2 domain and a short tail. It associates with the signaling lymphocyte-activation molecule, thereby acting as an inhibitor of this transmembrane protein by blocking the recruitment of the SH2-domain-containing signal-transduction molecule SHP-2 to its docking site. This protein can also bind to other related surface molecules that are expressed on activated T, B and NK cells, thereby modifying signal transduction pathways in these cells. Mutations in this gene cause lymphoproliferative syndrome X-linked type 1 or Duncan disease, a rare immunodeficiency characterized by extreme susceptibility to infection with Epstein-Barr virus, with symptoms including severe mononucleosis and malignant lymphoma. Multiple transcript variants encoding different isoforms have been found for this gene.

Alternate Names

DSHP, EBVS, IMD5, LYP, MTCP1, XLP, XLPD

Entrez Gene IDs

4068 (Human)

Gene Symbol

SH2D1A

OMIM

300 (Human)

UniProt

AAH20732 (Human)

Additional SH2D1A Products

Product Documents for SH2D1A Antibody (1C9) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for SH2D1A Antibody (1C9) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for SH2D1A Antibody (1C9) - Azide and BSA Free

Customer Reviews for SH2D1A Antibody (1C9) - Azide and BSA Free (1)

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Customer Images

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  • SH2D1A Antibody (1C9)
    Name: Amairelys Barroeta
    Application: Flow Cytometry
    Sample Tested: T cells
    Species: Human
    Verified Customer | Posted 12/10/2020
    Intracellular staining of human T cells with SH2D1A monoclonal antibody or isotype control antibody, followed by a Polyclonal Goat Anti-Mouse/RPE secondary antibody. Grey filled histogram: Isotype control, empty blue histogram: SH2D1A antibody.
    Intracellular Staining
    SH2D1A Antibody (1C9) - Azide and BSA Free H00004068-M01

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Protocols

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FAQs

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