Human SHBG ELISA Kit (Colorimetric)

Key Product Details

Product Specifications

Precision

Intra-Assay Precision (Precision within an assay) CV% 5.3%

Inter-Assay Precision (Precision between assays) CV% 8.975%

Recovery for Human SHBG ELISA Kit (Colorimetric)

Kit Contents for Human SHBG ELISA Kit (Colorimetric)

• Assay Buffer, contains preservative, ready to use.
• Control, contains preservative, ready to use. For control values and ranges please refer to vial label or QC-Datasheet.
• Enzyme Conjugate, ready to use, anti-SHBG antibody conjugate to horseradish peroxidase, contains preservative.
• Microtiterwells: Wells coated with anti-SHBG antibody (monoclonal).
• Note: The standards are calibrated against human SHBG, WHO standard (NIBSC 08/266).
• Standard A (0 nmol/L), contains preservative, ready to use.
• Standard B (4 nmol/L), contains preservative, ready to use.
• Standard C (16 nmol/L), contains preservative, ready to use.
• Standard D (65 nmol/L), contains preservative, ready to use.
• Standard E (260 nmol/L), contains preservative, ready to use.
• Stop Solution, ready to use, contains 0.5 M H2SO4. Avoid contact with the stop solution. It may cause skin irritation and burns.
• Substrate Solution, ready to use, tetramethylbenzidine (TMB).
• Wash Solution, 40X concentrated solution.

Preparation and Storage

Background: SHBG

The gene encodes an EF hand-containing small calcium binding protein. It is an integral subunit component of Kv4/D (Shal)-type voltage-gated rapidly inactivating A-type potassium channels. Kv4 potassium channels regulate action potentials in neurons and cardiac myocytes. The coexpression of ABP with pore-forming Kv4 alpha subunits causes changes in the gating and amplitude of Kv4 currents, thus profoundly affect the intracellular trafficking and molecular properties of Kv4.2 alpha subunits. It also cause a dramatic redistribution of Kv4.2, releasing intrinsic endoplasmic reticulum retention and allowing for trafficking to the cell surface. It may regulate A-type currents, and hence neuronal excitability, in response to changes in intracellular calcium. It maps to 5q35.1 region of human chromosome. Alternative splicing results in multiple transcript variant encoding different isoforms. Isoform 1 and isoform 2 are expressed in brain and kidney.

Additional SHBG Products

• All Products for SHBG
• SHBG ELISA Kits
• SHBG Primary Antibodies
• SHBG Proteins and Enzymes

Product Documents for Human SHBG ELISA Kit (Colorimetric)