Key Product Details

Species Reactivity

Mouse

Applications

Western Blot, Flow Cytometry, CyTOF-ready

Label

Janelia Fluor 525

Antibody Source

Monoclonal Rat IgG2A Clone # 645608
View all Siglec-1/CD169 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Siglec-1/CD169
Thr20-Leu1639 (predicted)
Accession # Q62230

Specificity

Detects mouse Siglec-1/CD169 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human Siglec-1, -5, -6, -7, -8, -9, -10, -11, -14, recombinant mouse Siglec-2, -3, -E, -F, -G, or -H is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Applications for Siglec-1/CD169 Antibody (645608) [Janelia Fluor® 525]

Application
Recommended Usage

CyTOF-ready

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer
Spectra Viewer

Flow Cytometry Panel Builder

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Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

0

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: Siglec-1/CD169

The siglecs are a family of membrane bound lectins (of the immunoglobulin superfamily) that bind sialic acid and mediate cell-cell interactions. Family members include sialoadhesin, CD22 and CD33. Sialoadhesin is a macrophage-specific cell adhesion molecule that is expressed on activated macrophages in chronic inflammation and in tumours.

Long Name

Sialic Acid Binding Ig-like Lectin 1

Alternate Names

CD169, Siglec1

Gene Symbol

SIGLEC1

Additional Siglec-1/CD169 Products

Product Documents for Siglec-1/CD169 Antibody (645608) [Janelia Fluor® 525]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Siglec-1/CD169 Antibody (645608) [Janelia Fluor® 525]



Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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