|Detection of Siglec‑1/CD169 in RAW 264.7 Mouse Cell Line by Flow Cytometry. RAW 264.7 mouse monocyte/macrophage cell line was stained with Mouse Siglec‑1/CD169 Monoclonal Antibody (Catalog # MAB5610, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113).|
|Detection of Mouse Siglec‑1/CD169 by Western Blot. Western blot shows lysates of mouse splenocytes. PVDF Membrane was probed with 1 µg/mL of Mouse Siglec‑1/CD169 Monoclonal Antibody (Catalog # MAB5610) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Siglec‑1/CD169 at approximately 180 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 1.|
Siglec-1, also known as sialoadhesin or CD169, is a 175‑185 kDa type I transmembrane glycoprotein belonging to the Siglec family of sialic acid specific I-type lectins within the immunoglobulin superfamily. Mouse Siglec-1 contains a 1619 amino acid (aa) extracellular domain (ECD) with one Ig-like V-set domain and 16 Ig-like C2-set domains. The ECD shares 73% and 83% aa sequence identity with human and rat Siglec-1, respectively. Alternate splicing generates two soluble isoforms containing either 16 or the first 3 Ig-like domains. Siglec-1 is expressed by some tissue macrophages, dendritic cells and circulating monocytes during certain infections. It binds sialylated molecules including MMR, MGL1/CD301a, MUC1, PSGL-1 and CD43.
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