Detects mouse Siglec-1/CD169 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human Siglec-1, -5, -6, -7, -8, -9, -10, -11, -14, recombinant mouse Siglec-2, -3, -E, -F, -G, or -H is observed.
Monoclonal Rat IgG2A Clone # 645608
Protein A or G purified from hybridoma culture supernatant
Detection of Siglec‑1/CD169 in RAW 264.7 Mouse Cell Line by Flow Cytometry.
RAW 264.7 mouse monocyte/macrophage cell line was stained with Mouse Siglec‑1/CD169 Monoclonal Antibody (Catalog # MAB5610, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113).
Detection of Mouse Siglec‑1/CD169 by Western Blot.
Western blot shows lysates of mouse splenocytes. PVDF Membrane was probed with 1 µg/mL of Mouse Siglec‑1/CD169 Monoclonal Antibody (Catalog # MAB5610) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Siglec‑1/CD169 at approximately 180 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Siglec-1, also known as sialoadhesin or CD169, is a 175‑185 kDa type I transmembrane glycoprotein belonging to the Siglec family of sialic acid specific I-type lectins within the immunoglobulin superfamily. Mouse Siglec-1 contains a 1619 amino acid (aa) extracellular domain (ECD) with one Ig-like V-set domain and 16 Ig-like C2-set domains. The ECD shares 73% and 83% aa sequence identity with human and rat Siglec-1, respectively. Alternate splicing generates two soluble isoforms containing either 16 or the first 3 Ig-like domains. Siglec-1 is expressed by some tissue macrophages, dendritic cells and circulating monocytes during certain infections. It binds sialylated molecules including MMR, MGL1/CD301a, MUC1, PSGL-1 and CD43.
Sialic Acid Binding Ig-like Lectin 1
Entrez Gene IDs:
6614 (Human); 20612 (Mouse); 311426 (Rat)
C19orf75; CD169; Siglec1; Siglec-1; SIGLECL1 SIGLEC family like 1
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We have 1 review tested in 1 application: Immunocytochemistry/Immunofluorescence.