Siglec-1/CD169 Antibody (ED3) - BSA Free

Novus Biologicals | Catalog # NB100-65301

Novus Biologicals
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Key Product Details

Species Reactivity

Rat

Applications

Immunohistochemistry, Immunohistochemistry-Frozen, Flow Cytometry, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # ED3

Format

BSA Free
View all Siglec-1/CD169 Primary Antibodies »

Product Specifications

Immunogen

Rat Spleen cell homogenate

Specificity

NB100-65301 recognizes the rat CD169 cell surface antigen, a 185kD molecule expressed by macrophages, predominately confined to lymphoid organs only. Monocytes and granulocytes are negative. No other cell types are positive. The most conspicious property of ED3 is it stains marginal zone macrophages and marginal metallophils in the spleen very strongly. Furthermore, macrophages in (auto-immune) diseased tissues express the ED3 antigen. In healthy tissue no expression occurs. CD169 is a receptor for glycoconjugates containing sialic acid.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Siglec-1/CD169 Antibody (ED3) - BSA Free

Immunohistochemistry-Frozen: Siglec-1/CD169 Antibody (ED3) - BSA Free [NB100-65301]

Immunohistochemistry-Frozen: Siglec-1/CD169 Antibody (ED3) - BSA Free [NB100-65301]

Immunohistochemistry-Frozen: Siglec-1/CD169 Antibody (ED3) [NB100-65301] - Rat lymph node cryosection with Mouse anti Rat CD169, clone ED3 followed by horseradish peroxidase conjugated Goat anti Mouse IgG2a as a detection reagent.

Applications for Siglec-1/CD169 Antibody (ED3) - BSA Free

Application
Recommended Usage

Flow Cytometry

1:500

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:50-1:250

Immunoprecipitation

1:10-1:500

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Siglec-1/CD169

Two families of mammalian lectin like adhesion molecules have been shown to bind glycoconjugate ligands in a sialic acid dependent manner: the selectins and the sialoadhesins. The sialoadhesin family has 4 members: CD22, a B cell specific marker; myelin associated glycoprotein (MAG), which is expressed on oligodendrocytes and Schwann cells; CD33, a myeloid differentiation antigen; and sialoadhesin, which is expressed only by a subpopulation of tissue macrophages. Involved in cell-cell interactions, sialoadhesin is structurally related to the 3 other listed members of the sialoadhesin family. CD169 is a sialic acid binding site of sialoadhesin. CD169 is a macrophage receptor expressed on stromal macrophages in many tissues, particularly found in lymph nodes, bone marrow and spleen.

Long Name

Sialic Acid Binding Ig-like Lectin 1

Alternate Names

CD169, Siglec1

Entrez Gene IDs

292844 (Rat)

Gene Symbol

SIGLEC1

Additional Siglec-1/CD169 Products

Product Documents for Siglec-1/CD169 Antibody (ED3) - BSA Free

Certificate of Analysis

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Product Specific Notices for Siglec-1/CD169 Antibody (ED3) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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