Simple Plex Human MMP-2 Cartridge

R&D Systems | Catalog # SPCKB-PS-000609

Simple Plex Cartridge Kit for quantitation of MMP-2. Includes wash buffer and sample diluent. For use with the Ella automated immunoassay system.
R&D Systems
Discontinued Product
SPCKB-PS-000609 has been discontinued. View all MMP-2 products.

Key Product Details

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Cell Culture Supernates (500 nL), Serum (500 nL), EDTA Plasma (500 nL), Heparin Plasma (500 nL)

Sensitivity

28.8 pg/mL

Assay Range

28.2-43070 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)

Simple Plex Human MMP-2 Cartridge Summary

Simple Plex assay for the detection of human MMP-2 in cell culture supernatant (CCS), serum, and plasma (EDTA/Heparin)

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View all MMP-2 Simple Plex »

Compatible Instruments

Ella Automated Immunoassay System

Ella Automated Immunoassay System

Efficiently analyze biomarkers with Ella's advanced technology, providing accurate, precise results in under 90 minutes. Quantify up to 8 targets per assay through a streamlined automated workflow. Choose from >350 highly validated Simple Plex assays or Simple Plex discovery assays.
Learn More

Product Specifications

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Species

Human

Specificity

Natural and recombinant human MMP-2.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 1 2
n 16 16 16 16
Mean (pg/mL) 212 13,746 250 12,409
Standard Deviation 18.3 1027 25.5 1627
CV% 8.6 7.5 10.2 13.1

Recovery for Simple Plex Human MMP-2 Cartridge

Recovery was not necessary as MMP-2 shows natural linearity data from a neat sample to a 1:16 dilution.

Linearity

Samples containing and/or spiked with high concentrations of human MMP-2 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay

Human MMP-2 Simple Plex Assay Linearity

Scientific Data Images for Simple Plex Human MMP-2 Cartridge

Human MMP-2 Simple Plex Assay Standard Curve

Human MMP-2 Simple Plex Assay Standard Curve

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: MMP-2

MMP-2 (matrix metalloproteinase 2), also known as Gelatinase A, is upregulated at sites of tissue damage, inflammation, and in stromal cells surrounding the invading front of metastatic tumors. The latent proenzyme (proMMP-2) is activated by MT-MMPs, Thrombin, or activated Protein C. Mature MMP-2 activity is inhibited by alpha 2-Macroglobulin or TIMPs 1, 2, 3, and -4. TIMP-2 and -3 can also tether proMMP-2 into cell surface ternary complexes with MT-MMPs. MMP-2 degrades Gelatin (denatured Collagen) and type IV Collagen, the major component of basement membranes. It can also degrade the matrix proteins Collagens V, VII and X, Decorin, Elastin, and Fibronectin. MMP-2 processes and modulates the functions of many other vasoactive and proinflammatory molecules including Adrenomedullin, Big Endothelin-1, Calcitonin gene-related peptide, CCL7/MCP-3, CXCL12/SDF-1, Galectin-3, IGFBP-3, IL-1 beta, S100A8, and S100A9.

Long Name

Matrix Metalloproteinase 2

Alternate Names

Gelatinase A, MMP2

Entrez Gene IDs

4313 (Human); 17390 (Mouse)

Gene Symbol

MMP2

Additional MMP-2 Products

Product Documents for Simple Plex Human MMP-2 Cartridge

Certificate of Analysis

To download a Certificate of Analysis, please enter your Simple Plex catalog number and lot number in the search boxes below.

Product Specific Notices for Simple Plex Human MMP-2 Cartridge

For research use only

Citations for Simple Plex Human MMP-2 Cartridge

View all citations for the Simple Plex platform.

Please visit our Instrument Citation Database to search our collection of scientific articles that feature our instruments.

Customer Reviews for Simple Plex Human MMP-2 Cartridge

There are currently no reviews for this product. Be the first to review Simple Plex Human MMP-2 Cartridge and earn rewards!

Have you used Simple Plex Human MMP-2 Cartridge?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card