Simple Plex Human MMP-2 Cartridge

Name: Simple Plex Human MMP-2 Cartridge
Brand: R&D Systems
SKU: SPCKB-PS-000609

Catalog #: SPCKB-PS-000609 Datasheet / COA / SDS

Simple Plex Cartridge Kit for quantitation of MMP-2. Includes wash buffer and sample diluent. For use with the Ella automated immunoassay system

Discontinued Product

SPCKB-PS-000609 has been discontinued.

View all MMP-2 products.

3 Images

All MMP-2 Simple Plex Assays

Product Details

FAQs

Reviews

Summary Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Simple Plex Human MMP-2 Cartridge Summary

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Cell Culture Supernates (500 nL), Serum (500 nL), EDTA Plasma (500 nL), Heparin Plasma (500 nL)

Sensitivity

28.8 pg/mL

Assay Range

28.2 - 43,070 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)

Specificity

Natural and recombinant human MMP-2.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

Product Summary

Simple Plex assay for the detection of human MMP-2 in cell culture supernatant (CCS), serum, and plasma (EDTA/Heparin)

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	16	16
Mean (pg/mL)	212	13746	250	12409
Standard Deviation	18.3	1027	25.5	1627
CV%	8.6	7.5	10.2	13.1

Recovery

Recovery was not necessary as MMP-2 shows natural linearity data from a neat sample to a 1:16 dilution.

Linearity

Samples containing and/or spiked with high concentrations of human MMP-2 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay

Scientific Data

N/A

View Larger

Human MMP-2 Simple Plex Assay Standard Curve

Product Datasheets

Product Datasheet

COA

SDS

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: MMP-2

The matrix metalloproteinases (MMPs) consist of 24 known human zinc proteases with essential roles in breaking down components of the extracellular matrix (ECM). Additional MMP substrates include cytokines, chemokines, growth factors and binding proteins, cell/cell adhesion molecules, and other proteinases. With a few exceptions, MMPs share common structural motifs including a pro-peptide domain, a catalytic domain, a hinge region, and a hemopexin-like domain. Synthesized as pro-enzymes, most MMPs are secreted before conversion to their active form. MMP activities are modulated on several levels including transcription, pro-enzyme activation, or by their endogenous inhibitors, tissue inhibitors of metalloproteinases (TIMPs). A subset of MMPs are associated with membranes and designated as membrane-type metalloproteinases (MT-MMP).

Long Name:

Matrix Metalloproteinase 2

Entrez Gene IDs:

4313 (Human); 17390 (Mouse)