Smad2 [p Ser467] Antibody - BSA Free
Novus Biologicals | Catalog # NBP2-54771
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Human
Applications
Validated:
Western Blot, Simple Western
Cited:
Immunohistochemistry-Paraffin, Western Blot, Simple Western
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
Smad2 (Phospho-Ser467) antibody was raised against a peptide sequence around phosphorylation site of serine 467 (C-S-S-M-S (p) ) derived from Human Smad2.
Modification
p Ser467
Specificity
This antibody detects endogenous level of Smad2 only when phosphorylated at serine 467.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
60 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Smad2 [p Ser467] Antibody - BSA Free
Immunocytochemistry/ Immunofluorescence: Smad2 [p Ser467] Antibody - BSA Free [NBP2-54771] -
Differences in muscle-to-collagen ratio and Smad pathway proteins in control and ESLUTD bladder. (A) Masson’s trichrome staining in control and ESLUTD bladder detrusor muscle with smooth muscle tissue (red) and connective tissue (blue). (B) Quantification of muscle-to-collagen ratio in control (n = 4) and ESLUTD (n = 4) smooth muscle tissue, normalized to control taken as 100%. Muscle-to-collagen ratio was significantly lower in ESLUTD compared to control bladder. Statistics with unpaired t-test, ** p ≤ 0.01. (C) Antibody staining for myostatin (red) and its signaling pathway proteins Smad 2 (green), p-Smad 2 (green) and follistatin (green) with cell nuclei in blue. Scale bar, 100 μm. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36901894), licensed under a CC-BY license. Not internally tested by Novus Biologicals.Applications for Smad2 [p Ser467] Antibody - BSA Free
Application
Recommended Usage
Western Blot
1:500-1:1000
Application Notes
See Simple Western Antibody Database for Simple Western validation: Tested in Bladder, separated by Size
Formulation, Preparation, and Storage
Purification
Epitope affinity purified
Formulation
PBS (pH 7.4) (without Mg2+ and Ca2+), 150mM NaCl, 50% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 °C.
Background: Smad2
Long Name
Mothers Against DPP Homolog 2
Alternate Names
JV18-1, MADH2, MADR2, Smox, pSMAD
Entrez Gene IDs
NP_001003652.1 (Human)
Gene Symbol
SMAD2
Additional Smad2 Products
Product Documents for Smad2 [p Ser467] Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Smad2 [p Ser467] Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Citations for Smad2 [p Ser467] Antibody - BSA Free
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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