Recombinant Human SNAP25 Protein
Novus Biologicals | Catalog # NBC1-18344
![SDS-PAGE: Recombinant Human SNAP25 Protein [NBC1-18344]](https://resources.rndsystems.com/images/products/SNAP25-Protein-SDS-Page-NBC1-18344-img0001.jpg "SDS-PAGE: Recombinant Human SNAP25 Protein [NBC1-18344]")
Product Specifications
Description
Source: E.coli
Amino Acid Sequence: MAEDADMRNE LEEMQRRADQ LADESLESTR RMLQLVEESK DAGIRTLVML DEQGEQLERI EEGMDQINKD MKEAEKNLTD LGKFCGLCVC PCNKLKSSDA YKKAWGNNQD GVVASQPARV VDEREQMAIS GGFIRRVTND ARENEMDENL EQVSGIIGNL RHMALDMGNE IDTQNRQIDR IMEKADSNKT RIDEANQRAT KMLGSG
Purity
Predicted Molecular Mass
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Protein / Peptide Type
Scientific Data Images for Recombinant Human SNAP25 Protein
SDS-PAGE: Recombinant Human SNAP25 Protein [NBC1-18344]
SDS-Page: SNAP25 Protein [NBC1-18344] - SNAP25, 23 kDa (206 aa), confirmed by MALDI-TOF with a purity of 90% by SDS - PAGEFormulation, Preparation, and Storage
NBC1-18344
| Formulation | 25mM Tris-HCl buffer (pH 7.5), 1 mM DTT, 10% glycerol |
| Preservative | No Preservative |
| Concentration | 1 mg/ml |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
Background: SNAP25
Long Name
Alternate Names
Gene Symbol
Additional SNAP25 Products
Product Documents for Recombinant Human SNAP25 Protein
Certificate of Analysis
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Product Specific Notices for Recombinant Human SNAP25 Protein
This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.
Related Research Areas
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FAQs for Recombinant Human SNAP25 Protein
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Q: I use your SNAP-25 protein routinely in my experiments using a frozen stock that I keep at -80C. It works fine. However, for my experiments I cleave the SNAP-25 (lop off the last 10 aa or so) and use this protein for my ELISA. I cleave the SNAP-25 by incubating it for 2 hours with the 50KD Enzymatic light chain from Botulinium toxin A. The fresh cleaved SNAP-25 works fine in my ELISA. When I take 0.5 mg/ml of the cleaved SNAP-25 (also containing 0.25 mg/ml toxin A) and freeze it at -80C in 25 mM Tris-HCL, 1 mM DTT, 2 mM EDTA, and 10% glycerol (your formulation for SNAP-25) I lose 70% of my activity in 3 days. Do you have any tips on how to store this cleaved SNAP-25? Preparing the product fresh every time is not practical!
A: I imagine that the loss of activity comes from storing the protein in the presence of Botulinum toxin A and not from freeze thawing. Have you tested that activity remains after a round of freezing and thawing in the absence of the toxin? In this situation, I think you need to remove the Botulinum toxin since even at -80C, the toxin could be continuing to cleave and destroy the protein. The toxin you said is 50kDa, and the protein is 25kDa. Could you remove the toxin by size exclusion chromatography? Additionally, I found this paper, Procedures for the Inactivation and Safe Containment of Toxins (http://www.ouhsc.edu/ehso/toxinproc.pdf) that suggests that you can inactivate the toxin by heating or by adding sodium hypochorite and/or sodium hydroxide. I know this protein can be used for Western blot, so it should be able to survive boiling but 200 degrees Celsius may be too much but could be worth trying on an aliquot? Size exclusion chromatography, bleaching and heating are our three suggestions at the moment. If none of these work, then you may have to continue to prepare it fresh.