Recombinant SARS-CoV-2 Spike RBD (K417T, E484K, N501Y) His (C-Term) Protein

Novus Biologicals | Catalog # NBP3-07925

Novus Biologicals
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Key Product Details

Source

HEK293

Tag

His (C-Term)
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Product Specifications

Description

A recombinant protein with a C-terminal His-tag corresponding to SARS-CoV-2 Spike RBD Protein with mutations of K417T, E484K, and N501Y.

(Accession #: YP_009724390.1(E484K, K417T, N501Y))

Use a manual defrost freezer and avoid repeated freeze thaw cycles.

Purity

>90%, by SDS-PAGE

Predicted Molecular Mass

35 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Formulation, Preparation, and Storage

NBP3-07925
Formulation PBS, pH 7.5
Preservative No Preservative
Concentration Please see the vial label for concentration. If unlisted please contact technical services.
Reconstitution Please reconstitute the protein with ddH2O to the concentration 1 mg/ml.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at 4C short term. Store at -20C long term. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Spike RBD

The coronavirus Spike protein receptor binding domain (RBD) resides within the S1 subunit and is responsible for binding to host cell receptors and initiating viral infection. Past coronaviruses SARS and MERS along with the global pandemic caused by SARS-CoV-2 have sparked great interest and scientific discovery leading to new vaccines and drug development. At the heart of coronavirus biology lies the Spike protein and its receptor binding domain. The Spike RBD is a 26 kDa domain consisting of a twisted five-stranded antiparallel beta sheet and sits at the apex of each Spike protein monomer. The Spike RBD is flexible thanks to a hinge region that allows for conformational changes that expose (up or open conformation) or hide (down or closed conformation) its receptor contacts. For SARS-CoV-2, the Spike RBD recognizes and tightly binds to the human receptor ACE-2. In the closely related MERS coronavirus, human DPPIV/CD26 acts as the receptor.

Long Name

Spike Receptor Binding Domain

Alternate Names

Brazil variant (501Y.V3)

Additional Spike RBD Products

Product Documents

Certificate of Analysis

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Product Specific Notices

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

Related Research Areas

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FAQs

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