STAG2 Antibody

Novus Biologicals | Catalog # NBP1-51992

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Bovine (100%), Canine (100%), Porcine (100%). Backed by our 100% Guarantee.

Applications

Western Blot, Peptide ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all STAG2 Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-SRGSTVRSKKSKPST corresponding to internal region according to NP_006594.3.

Epitope

C-SRGSTVRSKKSKPST

Specificity

This antibody is expected to recognize both reported isoforms (NP_001036214.1; NP_006594.3). Reported variants NP_001036215.1 and NP_001036214.1 represent identical protein: Reported variants NP_001036216.1 and NP_006594.3 represent identical protein.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for STAG2 Antibody

Western Blot: STAG2 Antibody [NBP1-51992]

Western Blot: STAG2 Antibody [NBP1-51992]

Western Blot: STAG2 Antibody [NBP1-51992] - (0.03ug/ml) staining of K562 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Applications for STAG2 Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:32000

Western Blot

0.1 - 0.3 ug/ml
Application Notes
WB: Approx. 160 kDa band observed in K562 lysates, ~140 kDa band in roden spleen, and ~150 kDa band in mouse thymus lysates (calculated MW of 146 kDa band according to human NP_001036214.1 and 140 kDa band according to mouse NP_001071180.1).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: STAG2

SA2 is a component of the cohesin complex, which is required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which, sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis. The subunit interacts directly with RAD21 in cohesin complex. Cohesin complexes are composed of a heterodimer between a SMC1 protein (SMC1L1 or SMC1L2) and SMC3, which are attached via their hinge domain, and RAD21 links them at their heads, and one STAG protein (STAG1, STAG2 or STAG3). In cohesin complexes, STAG2 is mutually exclusive with STAG1 and STAG3.

Alternate Names

bA517O1.1, cohesin subunit SA-2, DKFZp686P168, SA-2, SA2FLJ25871, SCC3 homolog 2, stromal antigen 2DKFZp781H1753

Entrez Gene IDs

10735 (Human); 20843 (Mouse); 313304 (Rat)

Gene Symbol

STAG2

UniProt

NP_001036214.1 (Human)

Additional STAG2 Products

Product Documents for STAG2 Antibody

Certificate of Analysis

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Product Specific Notices for STAG2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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