TFAX 488, TFP

Catalog # Availability Size / Price Qty
6621/1
Fluorescent Dyes Small Molecule
1 Image
Description: Green fluorescent dye; amine reactive

Chemical Name: 3,6-Diamino-9-[2-carboxy-4-[(2,3,5,6-tetrafluorophenoxy)carbonyl]phenyl]-4,5-disulfoxanthylium bis(triethylammmonium) salt

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Biological Activity

Amine reactive green fluorescent dye. Insensitive to pH in the range 4 - 10. Forms bright and photostable conjugates with proteins and antibodies. Less susceptible to spontaneous hydrolysis during conjugation reactions compared to SE or NHS dyes. Also displays greater stability compared to NHS and SE versions, typically lasting several hours. Suitable for use in flow cytometry, two-photon excitation microscopy, and super resolution mircoscopy techniques, such as dSTORM, SIM and STED and two photon excitation (TPE) microscopy. Excitation maximum = 495 nm; emission maximum = 515 nm; extinction coefficient = 73,000 M-1cm-1; quantum yield = 0.92.

TFAX 568, SE Optical Data

Emission color Green
Brightness
λabs 495 nm
λem 515 nm
Extinction coefficient (ε) 73,000 M-1cm-1
Quantum yield (φ) 0.92
Closest Laser line 488 nm
Alternative for Alexa Fluor® 488 TFP ester, AF 488, FITC, Cy2

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company.


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Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

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Technical Data

M.Wt:
884.91
Formula:
C27H14F4N2O11S2.C12H30N2
Solubility:
Soluble in DMSO
Storage:
Store at -20°C
CAS No:
878546-79-3

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.

Background References

  1. On the seeding and oligomerization of pGlu-amyloid peptides (in vitro).
    Schilling et al.
    Biochemistry, 2006;45:12393

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