TFAX 488, TFP

Tocris Bioscience | Catalog # 6621

Green fluorescent dye; amine reactive
Tocris Bioscience
Discontinued Product
6621 has been discontinued. View all TFAX Dyes products.

Key Product Details

Description

Green fluorescent dye; amine reactive

Wavelength

Excitation = 495 nm, Emission = 515 nm
View all TFAX Dyes »

Product Description

TFAX 488, TFP is an amine reactive green fluorescent dye. Insensitive to pH in the range 4 - 10. Forms bright and photostable conjugates with proteins and antibodies. Less susceptible to spontaneous hydrolysis during conjugation reactions compared to SE or NHS dyes. Also displays greater stability compared to NHS and SE versions, typically lasting several hours. Suitable for use in flow cytometry, two-photon excitation microscopy (TPE), and super resolution microscopy techniques, such as dSTORM, SIM and STED. Excitation maximum = 495 nm; emission maximum = 515 nm; extinction coefficient = 73,000 M-1cm-1; quantum yield = 0.92.

Optical Data

TFAX 488, TFP Dye Spectra
Emission Color Green
Brightness
λabs 495 nm
λem 515 nm
Extinction Coefficient (ε) 73,000 M-1cm-1
Quantum Yield (φ) 0.92
Closest Laser line 488 nm
Reactive Group TFP
Reactivity Primary amines
Cell Permeable No
pH Sensitivity pH insensitive pH 4-10
Application flow cytometry, two-photon excitation microscopy, dSTORM, SIM, STED
Alternative For Alexa Fluor® 488 TFP ester, AF 488, FITC, Cy2

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Product Specifications for TFAX 488, TFP

Molecular Weight

884.91

Formula

C27H14F4N2O11S2.C12H30N2

Storage

Store at -20°C

Chemical Name

3,6-Diamino-9-[2-carboxy-4-[(2,3,5,6-tetrafluorophenoxy)carbonyl]phenyl]-4,5-disulfoxanthylium bis(triethylammmonium) salt

CAS Number

878546-79-3

SMILES

CCN(CC)CC.CCN(CC)CC.[NH2+]=C1C=CC2=C(C3=CC=C(C(OC4=C(F)C(F)=CC(F)=C4F)=O)C=C3C([O-])=O)C5=CC=C(N)C(S(=O)(O)=O)=C5OC2=C1S(=O)(O)=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

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Background References

References are publications that support the biological activity of the product.

Product Documents for TFAX 488, TFP

Certificate of Analysis

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Product Specific Notices for TFAX 488, TFP

For research use only

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