Tissue Glue Kit

Immunohistochemistry

Kit

Protocol
1. This procedure will work for both frozen tissue sections and paraffinembedded tissue sections.
2. Sections should be mounted on positively-charged glass slides for best results.
3. For paraffin-embedded sections, the slides should be deparaffinized and rehydrated using standard procedures for deparaffinization.
4. Place slides into a water bath for five minutes
5. Remove slides from water bath and carefully remove excess water by wiping around the tissue with an absorbent wipe.
6. Lay the slides flat onto a level surface and pipette 200 ul of Reagent 1 onto the slides, making sure that the tissue is completely covered with reagent.
7. Incubate slides for 10 minutes at room temperature.
8. Drain off Reagent 1 by tipping the slides onto an absorbent towel and allowing reagent to run off the slides into the towel. Do not rinse slides.
9. Lay the slides flat onto a level surface and pipette 200 ul of Reagent 2 onto the slides, making sure that the tissue is completely covered with reagent.
10. Incubate slides for 10 minutes at room temperature.
11. Drain off Reagent 2 by tipping the slides onto an absorbent towel and allow reagent to run off of the slides into the towel. Do not rinse slides.
12. Lay the slides flat onto a level surface and allow slides to completely dry. Drying time can be shortened by drying slides in a 37 C incubator.
13. The polymers contained in these reagents prevent drying artifacts, and dried slides can be stored for several days before staining.
14. At the time of staining, place the slides into an appropriate antigen retrieval solution. The tissue glue will be removed from the slides during the antigen retrieval process.
15. After antigen retrieval commence immunohistochemical staining.

Concentration is not relevant for this product. Please see the protocols for proper use of this product.

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Store at 4C.