TMUB2 Antibody - Azide and BSA Free
Novus Biologicals | Catalog # NBP3-48418
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Key Product Details
Species Reactivity
Human
Applications
Western Blot, ELISA, Immunoprecipitation
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
Azide and BSA Free
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Product Specifications
Immunogen
Produced in rabbits immunized with purified, recombinant human TMUB2 (RefSeq#: NP_803190.2; Tyr37-Ser245).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C.
Scientific Data Images for TMUB2 Antibody - Azide and BSA Free
Western Blot: TMUB2 Antibody - Azide and BSA Free [NBP3-48418] -
Western Blot: TMUB2 Antibody - Azide and BSA Free [NBP3-48418] - Lane A: HeLa Whole Cell Lysate. Lane B: HepG2 Whole Cell Lysate. Lysates/proteins at 30 ug per lane using NBP3-48418 at 1:500 dilution. Secondary: HRP-conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 34 kDa. Observed band size: 34 kDaImmunoprecipitation: TMUB2 Antibody - Azide and BSA Free [NBP3-48418] -
Immunoprecipitation: TMUB2 Antibody - Azide and BSA Free [NBP3-48418] - Lane A: 0.5 mg HeLa Whole Cell Lysate. Lane B: 0.5 mg HepG2 Whole Cell Lysate. Blotted with 4 uL anti-TMUB2 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Immunoprecipitated using NBP3-48418 at 1:100 dilution and HRP-conjugated secondary antibody at 1:1000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 34 kDa. Observed band size :38 kDaApplications for TMUB2 Antibody - Azide and BSA Free
Application
Recommended Usage
ELISA
1:5000-1:10000
Immunoprecipitation
1-5 uL/mg of lysate
Western Blot
1:500-1:2000
Formulation, Preparation, and Storage
Purification
Antigen and protein A Affinity-purified
Formulation
0.2 um filtered solution in PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Store at -20C long term. Avoid freeze-thaw cycles.
Background: TMUB2
Alternate Names
MGC3123, transmembrane and ubiquitin-like domain containing 2, transmembrane and ubiquitin-like domain-containing protein 2
Gene Symbol
TMUB2
Additional TMUB2 Products
Product Documents for TMUB2 Antibody - Azide and BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for TMUB2 Antibody - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- Immunoprecipitation Protocol
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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