Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse

Cited:

Human

Applications

Validated:

Western Blot, Immunoprecipitation

Cited:

Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all TopBP1 Primary Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 1400 and the C-terminus (residue 1435) of human Topoisomerase (DNA) II Binding Protein 1 using the numbering given in entry NP_008958.1 (GeneID 11073).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for TopBP1 Antibody - BSA Free

Western Blot: TopBP1 Antibody [NB100-217]

Western Blot: TopBP1 Antibody [NB100-217]

Western Blot: TopBP1 Antibody [NB100-217] - Detection of mouse TopBP1 by western blot. Samples: Whole cell lysate (50 ug) from TCMK-1 and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-TopBP1 antibody NB100-217 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Western Blot: TopBP1 Antibody [NB100-217]

Western Blot: TopBP1 Antibody [NB100-217]

Western Blot: TopBP1 Antibody [NB100-217] - Sample: Whole cell lysate (50 ug for WB) from HeLa cells.
Western Blot: TopBP1 Antibody [NB100-217]

Western Blot: TopBP1 Antibody [NB100-217]

Western Blot: TopBP1 Antibody [NB100-217] - WB analysis of TOPBP1 in HeLa cell extract mitotic and interphase. Image courtesy of product review submitted by Jennifer Flynn.
Western Blot: TopBP1 Antibody [NB100-217]

Western Blot: TopBP1 Antibody [NB100-217]

Western Blot: TopBP1 Antibody [NB100-217] - Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-TOPBP1 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Western Blot: TopBP1 Antibody [NB100-217]

Western Blot: TopBP1 Antibody [NB100-217]

Western Blot: TopBP1 Antibody [NB100-217] - Detection of human TopBP1 by western blot. Samples: Whole cell lysate (15 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-TopBP1 antibody NB100-217 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Immunoprecipitation: TopBP1 Antibody [NB100-217]

Immunoprecipitation: TopBP1 Antibody [NB100-217]

Immunoprecipitation: TopBP1 Antibody [NB100-217] - Detection of human TopBP1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-TopBP1 antibody NB100-217 (lot NB100-217-2) used for IP at 3 ug per reaction. TopBP1 was also immunoprecipitated by a previous lot of this antibody (lot A300-111A-1). For blotting immunoprecipitated TopBP1, A300-111A was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

Applications for TopBP1 Antibody - BSA Free

Application
Recommended Usage

Immunoprecipitation

0.5 to 2.5 ug/mg of lysate

Reviewed Applications

Read 1 review rated 3 using NB100-217 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: TopBP1

Topoisomerases regulate topological states of DNA. DNA topoisomerase II-binding protein 1 (TopBP1) is a protein that interacts with the C-terminal region of topoisomerase II beta (Topo II beta). TopBP1 function has been found to be important to various aspects of cellular growth control such as DNA replication, the DNA damage checkpoint, and cell survival. TopBP1 interacts with several proteins through its BRCA1 C-terminal (BRCT) motifs. TopBP1 interacts and inhibits E2F1 transcriptional activity through an Rb-independent mechanism that involves recruitment of the SWI/SNF chromatin-remodeling complex proteins Brg1 and Brm. TopBP1 is also known as DNA topoisomerase 2-binding protein, DNA topoisomerase II beta-binding protein 1, TOPBP1, and KIAA0259.

Long Name

DNA Topoisomerase II Binding Protein 1

Alternate Names

TOP2BP1

Entrez Gene IDs

11073 (Human)

Gene Symbol

TOPBP1

UniProt

Q92547 (Human)

Additional TopBP1 Products

Product Documents for TopBP1 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for TopBP1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Cancer Biomarkers

Citations for TopBP1 Antibody - BSA Free

Customer Reviews for TopBP1 Antibody - BSA Free (1)

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  • Name: Anonymous
    Application: Western Blot
    Sample Tested: HeLa Cell Extract - Mitotic and Interphase, Sample Amount: Approximately 5x10^6 cell equivalents/lane
    Species: Human
    Verified Customer | Posted 07/21/2010
    TopBP1 Antibody - BSA Free NB100-217

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