TRIO Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-32088

Novus Biologicals
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Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Human

Applications

Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all TRIO Primary Antibodies »

Product Specifications

Immunogen

The immunogen this antibody was made to, maps to a region between residue 1575 to 1625 of human Trio Rho Guanine Nucleotide Exchange Factor using the numbering given in entry NP_009049.2 (GeneID 7204).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for TRIO Antibody - BSA Free

Western Blot: TRIO Antibody [NBP2-32088]

Western Blot: TRIO Antibody [NBP2-32088]

Western Blot: TRIO Antibody [NBP2-32088] - Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-TRIO antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Immunoprecipitation: TRIO Antibody [NBP2-32088]

Immunoprecipitation: TRIO Antibody [NBP2-32088]

Immunoprecipitation: TRIO Antibody [NBP2-32088] - Detection of human TRIO by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-TRIO antibody NBP2-32088 used for IP at 3 ug per reaction. TRIO was also immunoprecipitated by 2 other rabbit anti-TRIO antibodies. For blotting immunoprecipitated TRIO, NBP2-32088 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Applications for TRIO Antibody - BSA Free

Application
Recommended Usage

Immunoprecipitation

2 - 5 ug/mg lysate

Western Blot

1:1000 - 1:5000
Application Notes
Western blot of lysates performed using standard western blot reagents and 3-8% SDS-PAGE.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: TRIO

TRIO - triple functional domain (PTPRF interacting)

Alternate Names

ARHGEF23, EC 2.7.11.1, FLJ42780, PTPRF-interacting protein, tgat, triple functional domain (PTPRF interacting), triple functional domain protein

Gene Symbol

TRIO

UniProt

NP_009049.2 (Human)

Additional TRIO Products

Product Documents for TRIO Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for TRIO Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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