Recombinant Human TRPT1 His Protein

Novus Biologicals | Catalog # NBP1-78826

Novus Biologicals
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Key Product Details

Source

E. coli

Tag

His

Applications

SDS-PAGE
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Product Specifications

Description

A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-253 of Human TRPT1

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSHMNFSGG GRQEAAGSRG RRAPRPREQD RDVQLSKALS YALRHGALKL GLPMGADGFV PLGTLLQLPQ FRGFSAEDVQ RVVDTNRKQR FALQLGDPST GLLIRANQGH SLQVPKLELM PLETPQALPP MLVHGTFWKH WPSILLKGLS CQGRTHIHLA PGLPGDPGII SGMRSHCEIA VFIDGPLALA DGIPFFRSAN GVILTPGNTD GFLLPKYFKE ALQLRPTRKP LSLAGDEETE CQSSPKHSSR ERRRIQQ

Purity

>95%, by SDS-PAGE

Predicted Molecular Mass

30.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human TRPT1 His Protein

SDS-PAGE: Recombinant Human TRPT1 His Protein [NBP1-78826]

SDS-PAGE: Recombinant Human TRPT1 His Protein [NBP1-78826]

SDS-Page: Recombinant Human TRPT1 His Protein [NBP1-78826] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain

Formulation, Preparation, and Storage

NBP1-78826
Formulation 20 mM Tris-HCl buffer (pH8.0), 20% glycerol, 0.1 M NaCl, 1 mM DTT
Preservative No Preservative
Concentration 0.25 mg/ml
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TRPT1

TRPT1, also known as tRNA 2'-phosphotransferase 1, catalyzes the last step of tRNA splicing, the transfer of the splice junction 2'-phosphate from ligated tRNA to NAD to produce ADP-ribose 1''-2'' cyclic phosphate. Recombinant human TRPT1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

Alternate Names

EC 2.7.1.160, MGC11134, tRNA 2'-phosphotransferase 1, tRNA phosphotransferase 1, tRNA splicing 2' phosphotransferase 1

Gene Symbol

TRPT1

Additional TRPT1 Products

Product Documents for Recombinant Human TRPT1 His Protein

Certificate of Analysis

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Product Specific Notices for Recombinant Human TRPT1 His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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