TSA Vivid™ Fluorophore Kit 520

Catalog # Availability Size / Price Qty
7523/1 KIT
TSA Vivid™ Fluorophore Kit 520 | Tyramide Signaling Amplification/TSA
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Description: Signal amplification kit for use in ISH, ICC and IHC
Product Details

Biological Activity

TSA Vivid™ reagents are exceptionally bright dyes that offer an effective way to boost signal intensity and detect low-abundance targets in spatial biology applications. They are designed for use in ISH, IHC and ICC experiments where in situ detection of target protein or nucleic acid sequences is required. TSA Vivid reagents have been specifically optimized to deliver exceptional signal-to-noise in the RNAscope™ Multiplex Fluorescent v2 Assay for visualization of gene expression at the single cell level. They are suitable for multiplexing and can be combined with DAPI (Cat. No. 5748) counter-staining.

The basis for signal amplification comes from the specific and high-density deposition of TSA Vivid dyes adjacent to a horseradish peroxidase (HRP)-labeled probe in a process termed Tyramide Signal Amplification (TSA) or Catalyzed Reporter Deposition (CARD).

Excitation maximum = 494 nm; emission maximum = 517 nm

Please see the product datasheet or protocol for information on product use.

TSA Vivid is a trademark of Bio-Techne Corp.

Scientific Data

Application of TSA Vivid dye 520 in RNAscope Multiplex Fluorescent v2 Assay RNAscope Multiplex Fluorescent v2 Assay on HeLa cells with the leading competitor dye (left) and TSA Vivid dye 520 (right). Dye used at 1:1500 dilution. Marker shown is Polr2a in green. Nuclei are counterstained with DAPI (blue, Cat. No. 5748).

Optical Data

Spectral data for TSA Vivid Fluorophore kit 520 Emission Color Green
λabs 494 nm
λem 517 nm

Technical Data

Store at -20°C

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.

Product Datasheets

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Tocris Bioscience is the leading supplier of novel and exclusive tools for life science research with over 30 years' experience in the industry. Tocris is a Bio-Techne brand.