DAPI

Tocris Bioscience | Catalog # 5748

Blue-fluorescent DNA stain / dye. Used for: nuclear counterstain, fixed and live-cell staining, assessing apoptosis. Application: flow cytometry, confocal microscopy, immunofluorescence (IHC, ICC), ISH
Tocris Bioscience

Key Product Details

Description

Blue-fluorescent DNA stain / dye. Used for: nuclear counterstain, fixed and live-cell staining, assessing apoptosis. Application: flow cytometry, confocal microscopy, immunofluorescence (IHC, ICC), ISH

Alternative Names

DAPI Dye

Wavelength

Excitation = 350 nm, Emission = 470 nm
View all Fluorescent DNA Stains »

Product Description

Key information:

DAPI is a widely used blue-fluorescent DNA stain / dye. Membrane impermeant to live cells. For nuclear counterstain, fixed and live-cell staining (at high concentration).

Used for: nuclear counterstain, fixed and live-cell staining (at high concentration), DNA visualization, chromosome staining, DNA staining in agarose gels, apoptosis analysis, mouse embryos or fetal organs visualization.

Application: flow cytometry, confocal microscopy, immunofluorescence (IHC, ICC), and ISH.

Properties and Photophysical Data: DAPI stain binds to AT-rich regions of DNA and fluorescence increases by approximately 20-fold when bound to double-stranded DNA. It is reported to be more photostable than Hoechst 33342 (Cat. No. 5117). Suitable for multicolor fluorescence microscopy experiments. Excitation and emission maxima (λ) are 350 nm and 470 nm, respectively.

Scientific Data Images for DAPI

DAPI staining

DAPI staining.

HeLa cells were fixed for 10 min with 4% paraformaldehyde and permeabilized. DAPI was added at 0.1 μg/mL and stained cells were imaged with an LSM880 confocal microscope (Obj. 63X/1.4 oil). Scale bar = 10 μm.
DAPI counterstaining alongside MitoBrilliant™ Live 646

DAPI counterstaining alongside MitoBrilliant™ Live 646.

HeLa cells incubated with MitoBrilliant™ Live 646 (Catalog # 7417), 100 nM for 40 min. Cells were counterstained with DAPI. Image taken using an LSM880 Confocal using a 63X objective. Scale bar = 10 μm.

RNAScope™ Assay with DAPI Counterstain

RNAScope™ Assay with DAPI Counterstain.

3-plex RNAScope™ Multiplex Fluorescent v2 Assay on HeLa cells with ClariTSA™ dyes 520, 570 and 650 (Catalog # 7523, 7526 and 7527, Tocris). Nuclei are counter-stained with DAPI stain (blue). All dyes were used at 1:1500 dilution. Markers shown are Polr2a (green), PPIB (red) and UBC (white).

Optical Data

DAPI Dye Spectra
Emission Color Blue
λabs 350 nm
λem 470 nm
Closest Laser line 405 nm
Application Flow Cytometry, ISH, Confocal Microscopy, Immunofluorescence (IHC, ICC)

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer
Spectra Viewer

Product Specifications for DAPI

Molecular Weight

350.25

Formula

C16H15N5.2HCl

Storage

Store at -20°C

Purity

≥95% (HPLC)

Chemical Name

2-[4-(Aminoiminomethyl)phenyl]-1H-Indole-6-carboximidamide hydrochloride

CAS Number

28718-90-3

PubChem ID

160166

InChI Key

FPNZBYLXNYPRLR-UHFFFAOYSA-N

SMILES

N=C(N)C(C=C1)=CC=C1C2=CC3=CC=C(C(N)=N)C=C3N2.Cl.Cl

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
water 3.5 10 with gentle warming
DMSO 35.02 100

Preparing Stock Solutions for DAPI

The following data is based on the product molecular weight 350.25.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 2.86 mL 14.28 mL 28.55 mL
5 mM 0.57 mL 2.86 mL 5.71 mL
10 mM 0.29 mL 1.43 mL 2.86 mL
50 mM 0.06 mL 0.29 mL 0.57 mL

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Background References

References are publications that support the biological activity of the product. See our Citations tab to view 101 publications citing the usage of this product.

Product Documents for DAPI

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for DAPI

For research use only

Customer Reviews for DAPI (2)

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Customer Images

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  • DAPI staining of PC3 cells to detect apoptosis
    Name: Anonymous
    Species: Human
    Verified Customer | Posted 12/13/2018
    DAPI staining of PC3 cells treated with DR5 monoclonal antibodies to detect apoptosis and observing it with Floid Fluorescence Microscopy System.
    DAPI 5748
  • Excellent counter-stain...
    Name: Purnima Gupta
    Species: Mouse
    Assay Type: In Vivo
    Cell Line/Tissue: Brain
    Verified Customer | Posted 01/10/2018
    Used as counter stain in IHC

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