Catalog # Availability Size / Price Qty
DAPI | CAS No. 28718-90-3 | Fluorescent DNA Stains
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Description: Fluorescent DNA dye; DAPI can be used for cell staining and assessing apoptosis; suitable for flow cytometry; DAPI mw and DAPI ex/em, provided
Alternative Names: DAPI Dye

Chemical Name: 2-[4-(Aminoiminomethyl)phenyl]-1H-Indole-6-carboximidamide hydrochloride

Purity: ≥95%

Product Details
Citations (14)

Biological Activity

DAPI is a fluorescent DNA stain / dye. DAPI stain binds to AT-rich regions of DNA and fluorescence increases by approximately 20-fold when bound to double-stranded DNA. DAPI stain can be used in flow cytometry, DNA visualization and quantification in histochemistry/biochemistry, to assess apoptosis, and to visualize mouse embryos or fetal organs. DAPI stain can be used in fixed cells and is cell-permeable at high concentrations providing effective cell staining. It is reported to be more photostable than Hoechst 33342 (Cat. No. 5117). Em/Ex λ = 350/470 nm respectively.

Scientific Data

Confocal Microscopy DAPI staining View Larger

DAPI staining. HeLa cells were fixed for 10 min with 4% paraformaldehyde and permeabilized. DAPI was added at 0.1 μg/mL and stained cells were imaged with an LSM880 confocal microscope (Obj. 63X/1.4 oil). Scale bar = 10 μm.

Confocal Microscopy DAPI counterstaining alongside MitoBrilliant™ Live 646 View Larger

DAPI counterstaining alongside MitoBrilliant™ Live 646. HeLa cells incubated with MitoBrilliant™ Live 646 (Catalog # 7417), 100 nM for 40 min. Cells were counterstained with DAPI. Image taken using an LSM880 Confocal using a 63X objective. Scale bar = 10 μm.

Fluorescence Imaging RNAScope™ Assay with DAPI Counterstain View Larger

RNAScope™ Assay with DAPI Counterstain. 3-plex RNAScope™ Multiplex Fluorescent v2 Assay on HeLa cells with TSA Vivid™ dyes 520, 570 and 650 (Catalog # 7523, 7526 and 7527, Tocris). Nuclei are counter-stained with DAPI stain (blue). All dyes were used at 1:1500 dilution. Markers shown are Polr2a (green), PPIB (red) and UBC (white).

Optical Data for DAPI

DAPI Dye Spectra
Emission Color Blue
λabs 350 nm
λem 470 nm
Closest Laser line 405 nm
Application Flow Cytometry, ISH, Confocal Microscopy, Immunofluorescence (IHC, ICC)

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

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Technical Data

Soluble to 100 mM in DMSO and to 10 mM in water with gentle warming
Store at -20°C

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.

Product Datasheets

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*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and CoA (available online).

Citations for DAPI

The citations listed below are publications that use Tocris products. Selected citations for DAPI include:

14 Citations: Showing 1 - 10

  1. Intermittent compressive force regulates dentin matrix protein 1 expression in human periodontal ligament stem cells.
    Authors: Thanaphum Et al.
    J Dent Sci  2023;18:105-111
  2. Gut Microbiota-Derived Glutamine Attenuates Liver Ischemia/Reperfusion Injury via Macrophage Metabolic Reprogramming.
    Authors: Yu Et al.
    Cell Mol Gastroenterol Hepatol  2023;15:1255-1275
  3. BdLT-Seq as a barcode decay-based method to unravel lineage-linked transcriptome plasticity.
    Authors: Yelyzaveta Et al.
    Nat Commun  2023;14:1085
  4. Regulation of Cx43 Gap Junction Intercellular Communication by Bruton's Tyrosine Kinase and Interleukin-2-Inducible T-Cell Kinase.
    Authors: Paul L Et al.
    Biomolecules  2023;13
  5. Erythropoietin administration exerted neuroprotective effects against cardiac ischemia/reperfusion injury.
    Authors: Nipon Et al.
    Curr Res Pharmacol Drug Discov  2022;3:100124
  6. A mesenchymal to epithelial switch in Fgf10 expression specifies an evolutionary-conserved population of ionocytes in salivary glands.
    Authors: Marit H Et al.
    Cell Rep  2022;39:110663
  7. Modulation of mitochondrial dynamics rescues cognitive function in rats with 'doxorubicin-induced chemobrain' via mitigation of mitochondrial dysfunction and neuroinflammation.
    Authors: Titikorn Et al.
    FEBS J  2022;289:6435-6455
  8. Interplay of vascular endothelial growth factor receptors in organ-specific vessel maintenance.
    Authors: Satu Et al.
    J Exp Med  2022;219
  9. HSP70 chaperones RNA-free TDP-43 into anisotropic intranuclear liquid spherical shells.
    Authors: Digvijay Et al.
    Science  2021;371
  10. A protocol for rapid pericyte differentiation of human induced pluripotent stem cells.
    Authors: Elizabeth Et al.
    STAR Protoc  2021;2:100261


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