DAPI

Catalog # Availability Size / Price Qty
5748/10
5748/50
DAPI | CAS No. 28718-90-3 | Fluorescent DNA Stains
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Description: Fluorescent DNA dye; dapi can be used for cell staining and assessing apoptosis; suitable for flow cytometry; DAPI mw and DAPI ex/em, provided
Alternative Names: DAPI Dye

Chemical Name: 2-[4-(Aminoiminomethyl)phenyl]-1H-Indole-6-carboximidamide hydrochloride

Purity: ≥95%

Product Details
Reviews (2)

Biological Activity

DAPI is a fluorescent DNA stain / dye. DAPI binds to AT-rich regions of DNA. DAPI can be used in flow cytometry and to assess apoptosis. DAPI is cell permeable at high concentrations providing effective cell staining. Em/Ex λ = 350/470 nm respectively.

Optical Data for DAPI

λabs 350 nm
λem 470 nm
Application Flow cytometry

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

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Technical Data

M.Wt:
350.25
Formula:
C16H15N5.2HCl
Solubility:
Soluble to 100 mM in DMSO and to 10 mM in water with gentle warming
Purity:
≥95%
Storage:
Store at -20°C
CAS No:
28718-90-3

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.

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Reviews for DAPI

Average Rating: 4.5 (Based on 2 Reviews)

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DAPI staining of PC3 cells to detect apoptosis
By Anonymous on 12/13/2018
Application: Species: Human

DAPI staining of PC3 cells treated with DR5 monoclonal antibodies to detect apoptosis and observing it with Floid Fluorescence Microscopy System.


Excellent counter-stain...
By Purnima Gupta on 01/10/2018
Application: Species: Mouse

Used as counter stain in IHC


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