DAPI
Chemical Name: 2-[4-(Aminoiminomethyl)phenyl]-1H-Indole-6-carboximidamide hydrochloride
Purity: ≥95%
Biological Activity
DAPI is a fluorescent DNA stain / dye. DAPI binds to AT-rich regions of DNA. DAPI can be used in flow cytometry and to assess apoptosis. DAPI is cell permeable at high concentrations providing effective cell staining. Em/Ex λ = 350/470 nm respectively.Optical Data for DAPI
| λabs | 350 nm |
|---|---|
| λem | 470 nm |
| Application | Flow cytometry |
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Technical Data
The technical data provided above is for guidance only.
For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Background References
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A simple cytochemical technique for demonstration of DNA in cells infected with mycoplasmas and viruses.
Russell et al.
Nature, 1975;253:461 -
Interaction of 4'-6-diamidino-2-phenylindole to nucleic acids, and its implication to their template activity in RNA-polymerase reaction of E. coli bacteria and of Friend-virus infected mouse spleen.
Mildner et al.
Cancer Lett., 1978;4:89
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DAPI staining of PC3 cells treated with DR5 monoclonal antibodies to detect apoptosis and observing it with Floid Fluorescence Microscopy System.
Used as counter stain in IHC
