VAV2 Antibody (3F5D3) - BSA Free

Novus Biologicals | Catalog # NBP2-52583

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Flow Cytometry, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 3F5D3

Format

BSA Free
View all VAV2 Primary Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human VAV2 (AA: 552-868) expressed in E. Coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

101.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for VAV2 Antibody (3F5D3) - BSA Free

Western Blot: VAV2 Antibody (3F5D3)BSA Free [NBP2-52583]

Western Blot: VAV2 Antibody (3F5D3)BSA Free [NBP2-52583]

Western Blot: VAV2 Antibody (3F5D3) [NBP2-52583] - Analysis using VAV2 mAb against human VAV2 (AA: 552-868) recombinant protein. (Expected MW is *** kDa)
Flow Cytometry: VAV2 Antibody (3F5D3) - BSA Free [NBP2-52583]

Flow Cytometry: VAV2 Antibody (3F5D3) - BSA Free [NBP2-52583]

Flow Cytometry: VAV2 Antibody (3F5D3) [NBP2-52583] - Analysis of Hela cells using VAV2 mouse mAb (green) and negative control (red).
Western Blot: VAV2 Antibody (3F5D3)BSA Free [NBP2-52583]

Western Blot: VAV2 Antibody (3F5D3)BSA Free [NBP2-52583]

Western Blot: VAV2 Antibody (3F5D3) [NBP2-52583] - Western blot analysis using VAV2 mAb against HEK293 (1) and VAV2 (AA: 552-868)-hIgGFc transfected HEK293 (2) cell lysate.
ELISA: VAV2 Antibody (3F5D3) - BSA Free [NBP2-52583]

ELISA: VAV2 Antibody (3F5D3) - BSA Free [NBP2-52583]

ELISA: VAV2 Antibody (3F5D3) [NBP2-52583] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng).

Applications for VAV2 Antibody (3F5D3) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200-1:400

Western Blot

1:500-1:2000
Application Notes
This antibody is Cytof ready.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: VAV2

The Vav family are Rho/Rac guanosine nucleotide exchange factors (GEFs), consisting of three members in mammalian cells (Vav, Vav2, Vav3) and one in nematodes (CelVav) (1). Vav and Vav2 are members of the dbl family of guanine nucleotide exchange factors (GEF) for the rho/rac family of GTP binding proteins. Vav is expressed primarily in hematopoietic cells, while vav2 has a wider tissue distribution (2). Vav2 serves as a guanine nucleotide exchange factor for the Rho family of Ras-related GTPases. Unlike Vav1, whose expression is restricted to cells of hematopoietic origin, Vav2 is broadly expressed. Recently, Vav2 has been identified as a substrate for the epidermal growth factor (EGF) receptor. EGF receptor phosphorylates Vav2 on all three possible phosphorylation sites, Tyr-142, Tyr-159, and Tyr-172. results suggest that EGF regulates Vav2 activity basically through phosphatidylinositol 3-kinase activation, whereas tyrosine phosphorylation of Vav2 may rather be necessary for mediating protein-protein interactions (3)

Alternate Names

guanine nucleotide exchange factor VAV2, oncogene VAV2, Protein vav-2, vav 2 guanine nucleotide exchange factor, vav 2 oncogene, VAV-2

Gene Symbol

VAV2

Additional VAV2 Products

Product Documents for VAV2 Antibody (3F5D3) - BSA Free

Certificate of Analysis

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Product Specific Notices for VAV2 Antibody (3F5D3) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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