VPS29 Antibody

Novus Biologicals | Catalog # NB100-1387

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bovine (100%), Canine (100%), Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Western Blot, Peptide ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all VPS29 Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-DDVKVERIEYKKP corresponding to C-Terminus according to NP_057310.1, NP_476528.1.

Specificity

This antibody is expected to recognise both human isoforms (represented by NP_057310.1; NP_476528.1).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Theoretical MW

21 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for VPS29 Antibody

Western Blot: VPS29 Antibody [NB100-1387]

Western Blot: VPS29 Antibody [NB100-1387]

Western Blot: VPS29 Antibody [NB100-1387] - (0.1ug/ml) of Human Spleen lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Applications for VPS29 Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:16000

Western Blot

0.1 - 0.3 ug/ml
Application Notes
WB: Approx. 20+21 kDa band bands observed in human spleen lysates (calculated MW of 20.5 kDa band according to NP_057310.1 and 20.9 kDa band according to NP_476528.1).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: VPS29

VPS29 is an essential component of the retromer complex, a complex required to retrieve lysosomal enzyme receptors (IGF2R and M6PR) from endosomes to the trans-Golgi network. Also required to regulate transcytosis of the polymeric immunoglobulin receptor (pIgR-pIgA). Has low protein phosphatase activity towards a serine-phosphorylated peptide derived from IGF2R

Alternate Names

DC15, DC7, DKFZp564F0223, EC 3.1.3.3, FLJ20492, hVPS29, PEP11, PEP11 homolog, retromer protein, vacuolar protein sorting 29 (yeast homolog), vacuolar protein sorting 29 (yeast), vacuolar protein sorting 29 homolog (S. cerevisiae), vacuolar protein sorting-associated protein 29, vacuolar sorting protein VPS29/PEP11, Vesicle protein sorting 29, x 007 protein

Entrez Gene IDs

51699 (Human); 56433 (Mouse); 288666 (Rat)

Gene Symbol

VPS29

UniProt

Q9UBQ0 (Human)

Additional VPS29 Products

Product Documents for VPS29 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for VPS29 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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