Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Applications

Validated:

Knockout Validated, Western Blot, Immunoprecipitation

Cited:

Knockout Validated, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all WRN Primary Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residues 1400 and the C-terminus (residue 1432) of human Werner Syndrome Helicase using the numbering given in SwissProt entry Q14191 (GeneID 7486).

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 30971823).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

162 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for WRN Antibody - BSA Free

Western Blot: WRN Antibody [NB100-472]

Western Blot: WRN Antibody [NB100-472]

Western Blot: WRN Antibody [NB100-472] - Whole cell lysate (10 ug) from K-562, RKO, LNCaP, Jurkat, and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-WRN antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Western Blot: WRN Antibody [NB100-472]

Western Blot: WRN Antibody [NB100-472]

Western Blot: WRN Antibody [NB100-472] - Detection of human WRN on HeLa whole cell lysate using NB100-472. WRN was immunoprecipitated with NB100-472 or rabbit anti-WRN antibody NB100-471.

Applications for WRN Antibody - BSA Free

Application
Recommended Usage

Immunoprecipitation

2-5 ug/mg lysate

Western Blot

1:5000-1:25000

Reviewed Applications

Read 1 review rated 4 using NB100-472 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: WRN

This gene encodes a member of the RecQ subfamily and the DEAH (Asp-Glu-Ala-His) subfamily of DNA and RNA helicases. DNA helicases are involved in many aspects of DNA metabolism, including transcription, replication, recombination, and repair. This protein contains a nuclear localization signal in the C-terminus and shows a predominant nucleolar localization. It possesses an intrinsic 3' to 5' DNA helicase activity, and is also a 3' to 5' exonuclease. Based on interactions between this protein and Ku70/80 heterodimer in DNA end processing, this protein may be involved in the repair of double strand DNA breaks. Defects in this gene are the cause of Werner Syndrome, an autosomal recessive disorder characterized by premature aging.

Long Name

Werner syndrome ATP-dependent helicase

Alternate Names

EC 3.1.-.-, Exonuclease WRN, RecQ protein-like 2, RecQ3, RECQL2

Entrez Gene IDs

7486 (Human)

Gene Symbol

WRN

UniProt

Q14191 (Human)

Additional WRN Products

Product Documents for WRN Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for WRN Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for WRN Antibody - BSA Free

Customer Reviews for WRN Antibody - BSA Free (1)

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  • WRN Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Pancreas tissue, Stomach tissue, Skin tissue and Testis tissue
    Species: Canine and Feline
    Verified Customer | Posted 06/29/2017
    Lane 1: Canine Pancreas Lane 2: Canine Skin Lane 3: Canine Stomach Lane 4: Canine Testis Lane 5: Feline Pancreas Lane 6: Feline Skin Lane 7: Feline Stomach Lane 8: Feline Testis Bands are not present in the expected 162kDa range,
    This antibody will be used for IHC-P in both canine and feline tissue (results not yet available).
    WRN Antibody - BSA Free NB100-472

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