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X-Gal

5510 1 Review Datasheet / COA / SDS

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X-Gal | CAS No. 7240-90-6 | Buffers, Chelators and Reagents
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Description: Used in cloning procedures with IPTG

Chemical Name: 5-Bromo-4-chloro-1H-indoll-3-yl-β-D-galactopyranoside

Purity: ≥98%

Product Details
Reviews (1)
Biological Activity Technical Data Product Datasheets Calculators

Biological Activity

Chromogenic substrate of β-Galactosidase that produces blue color. Used in combination with IPTG (Cat. No. 5509) for detection of β-Galactosidase activity in bacterial colonies.

Technical Data

M.Wt:
408.63
Formula:
C14H15BrClNO6
Solubility:
Soluble to 100 mM in DMSO
Purity:
≥98%
Storage:
Store at -20°C
CAS No:
7240-90-6

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.

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X gal for Blue White Screening
By Anonymous on 02/04/2019
Application: Species: Human

Bacterial cells were plated with L-agar, Ampillicin and X-gal solution at two different concentrations (5 and 50 µl) and a negative control containing only sterile water was set up. The plates were allowed to grow for 48 hours and counted for white and blue colonies.

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