Chemical Name: 5-Bromo-4-chloro-1H-indoll-3-yl-β-D-galactopyranoside
Biological ActivityChromogenic substrate of β-Galactosidase that produces blue color. Used in combination with IPTG (Cat. No. 5509) for detection of β-Galactosidase activity in bacterial colonies.
The technical data provided above is for guidance only.
For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
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Bacterial cells were plated with L-agar, Ampillicin and X-gal solution at two different concentrations (5 and 50 µl) and a negative control containing only sterile water was set up. The plates were allowed to grow for 48 hours and counted for white and blue colonies.