Z-Leu-Arg-Gly-Gly-AMC (Z-LRGG-AMC)

R&D Systems | Catalog # S-100

R&D Systems
Discontinued Product
S-100 has been discontinued. View all Deubiquitinating Enzyme Substrates products.

Key Product Details

Source

Synthetic

Conjugate

7-Amino-4-Methyl Coumarin

Applications

Enzyme Activity
View all Deubiquitinating Enzyme Substrates Small Molecules and Peptides »

Formulation, Preparation, and Storage

S-100
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

Background: Deubiquitinating Enzyme Substrates

Fluorgenic tetra-peptide substrate for ubiquitin C-terminal hydrolases (UCHs, eg. UCHL3) and Isopeptidase T. This low molecular weight peptide represents a minimal sequence of C-terminal residues of ubiquitin where hydrolysis occurs at the UbGly76-X bond. The catalytic efficiency for hydrolysis of this substrate by deconjugating enzymes is several thousand-fold less than a more native substrate (Ub-AMC, Cat # U-550) since remote interactions with Ub-containing substrates stabilize catalytic transition states

References

  1. Dang L. C. et al. (1998) Biochemistry 37:1868 -1879
  2. Mayer A. N. and Wilkinson K. D. (1989) Biochemistry 28:166-172
  3. Stein R. L. et al. (1995) Biochemistry 34:12616 -12623
  4. Wilkinson K. D. et al. (1995) Biochemistry 34:14535-14546
  5. Wold W. S. M. and Tollefson A. E. (2008) Meth. Mol. Med. 131:251-255

Product Documents for Z-Leu-Arg-Gly-Gly-AMC (Z-LRGG-AMC)

Certificate of Analysis

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Product Specific Notices for Z-Leu-Arg-Gly-Gly-AMC (Z-LRGG-AMC)

For research use only

Related Research Areas

Deubiquitinating Enzymes (DUBs)

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