Characterization of Potent Wnt/R-Spondin Agonist Proteins for Research and Therapeutic Applications

To resolve the challenges of purifying hydrophobic Wnt proteins in an active state and using multiple proteins in a single experiment, R&D Systems™ has engineered a Wnt agonist protein that can act as a Wnt and R-Spondin pathway activator to promote canonical Wnt signaling. As seen in this application note, Wnt/R-Spondin Agonist Proteins display better activity in a Wnt/β-Catenin assay compared to Wnt-3a with or without R-Spondin proteins. Wnt/R-Spondin Agonist Proteins are ideal for organoids and 3D cell culture, as they support robust intestinal organoid culture at lower concentrations relative to Wnt-3a and R-Spondin 1 together.

Benefits of Wnt/R-Spondin Agonist Proteins:

All-in-One Activation: Reduce experimental complexity with a single, cost-effective protein
Streamline Workflows: Save time with a well-defined reagent to substitute for conditioned media or the addition of two individual proteins
Reliable Performance: Effectively promotes Wnt receptor activation and canonical Wnt signaling
Increased Solubility: More amenable to media formulations than traditional Wnt proteins