Stability and Dependability in Cell Manufacturing: The Development of an AI-Modified IL-2 Heat Stable Agonist Protein

To overcome challenges encountered when using cytokines for cell therapy applications, R&D Systems™ has used artificial intelligence (AI)/predictive analytics to engineer an IL-2 Heat Stable Agonist Protein. Designed for thermostability, this protein contains a stabilized synthetic core and enhanced affinity for the IL-2 dimeric βγ receptor. This application note shows the IL-2 Heat Stable Agonist Protein has a higher melting temperature, retains activity in various concentrations at 37˚C and 95˚C, and maintains T cell outgrowth similarly to the standard IL-2 protein. The improved stability and dependability of IL-2 Heat Stable Agonist Protein make it an ideal choice for the manufacture of immune-based therapies.

Benefits of IL-2 Heat Stable Agonist Protein:
  • Improved Stability: Able to withstand high temperatures and extended culture durations due to a synthetic core designed to strengthen the protein’s structure
  • Consistent Cultures: Increased stability in media over extended durations allows for greater consistency throughout cell cultures
  • CD122 Binding Affinity: Increased affinity to IL-2 beta receptor with decreased affinity to IL-2 alpha receptor promotes proliferation of effector T cells
  • Reliable Performance: Promotes CD8+/CD4+ T cell outgrowth and similar T cell phenotypes as the standard IL-2 protein
 Stability and Dependability in Cell Manufacturing
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