Troubleshooting Guide: MagCellect Cell Isolation Kits
This protocol is intended as a guide only.
Our MagCellect Cell Isolation kits are designed to rapidly isolate specific cell populations. We have attempted to make the kits complete and easy to use. To ensure that all users get optimal performance with these products we have summarized some common problems below. We attempt to identify the likely sources of these problems and possible courses of action to be taken to avoid them. We urge users to review these tips and if additional advice is required to contact our technical service department.
Problem: Low cell recovery
| Possible Source | Test or Action |
|---|---|
| Excessive or nonspecific binding of antibodies and/or ferrofluids to the desired cells | Follow the incubation times indicated and/or carry out reactions at the temperature indicated in the product insert |
| Non optimal number of cells used | Use the recommended ratio of cells and antibody mixture |
Problem: Low/poor cell purity
| Possible Source | Test or Action |
|---|---|
| Insufficient antibody used | Use the recommended ratio of cells and antibody mixture |
| Non-optimal amounts of ferrofluid used | Use the recommended ratio of cells and ferrofluids |
| Carryover of magnetically tagged cells in a negative selection procedure | Harvest un-tagged cells carefully without touching the tube wall where magnetically tagged cells are located |
| Magnetic cell separation reaction had not gone to completion | Perform the magnetic separation with the recommended magnet and for the recommended time |