Coupling Rates for 30 Minute Kinase Reactions

The following table lists the coupling rates for 30 minute kinase reactions coupled with Recombinant Mouse CD39L2. The coupling rates are for 50 μL reactions carried out at room temperature in a buffer consisting of 25 mM HEPES, 10 mM CaCl₂, 10 mM MgCl₂, 150 mM NaCl, pH 7.0. The coupling rates were calculated using a specific rate constant of 97.78 nmol/min/μg/mM.

NOTE: An * represents the optimal range for coupling rates in order to achieve the maximum signal-to-noise ratio for the phosphatase-coupled kinase reaction.

For more detailed technical information, please also see: Wu, Z.L. (2011) PLoS ONE 6(8):e23172.

Coupling Rates for 30 Minute Kinase Reactions

ATP (μM)	CD39L2 (μg)
ATP (μM)	0.025 μg	0.038 μg	0.050 μg	0.063 μg	0.075 μg	0.088 μg	0.100 μg	0.125 μg	0.150 μg	0.175 μg	0.200 μg
50 μM	0.431*	0.550*	0.634	0.696	0.741	0.776	0.802	0.841	0.868	0.886	0.901
100 μM	0.415*	0.533*	0.618	0.680	0.727	0.763	0.791	0.832	0.860	0.880	0.895
150 μM	0.403*	0.521*	0.606	0.669	0.717	0.754	0.783	0.825	0.854	0.875	0.890
200 μM	0.394	0.510*	0.596*	0.660	0.708	0.746	0.776	0.819	0.849	0.870	0.886
250 μM	0.386	0.502*	0.587*	0.652	0.701	0.739	0.769	0.814	0.844	0.866	0.883
300 μM	0.379	0.494*	0.580*	0.644	0.694	0.733	0.764	0.809	0.840	0.863	0.880
400 μM	0.367	0.481*	0.567*	0.632	0.682	0.722	0.754	0.801	0.833	0.857	0.875
500 μM	0.357	0.470*	0.556*	0.621	0.672	0.713	0.745	0.793	0.827	0.851	0.870
600 μM	0.349	0.461*	0.546*	0.612	0.664	0.705	0.738	0.787	0.821	0.847	0.866
700 μM	0.342	0.452*	0.537*	0.604	0.656	0.697	0.731	0.781	0.816	0.842	0.862
800 μM	0.335	0.445*	0.530*	0.596*	0.648	0.690	0.724	0.776	0.812	0.838	0.858
900 μM	0.329	0.438*	0.522*	0.589*	0.642	0.684	0.718	0.770	0.807	0.834	0.855
1000 μM	0.324	0.432*	0.516*	0.582*	0.635	0.678	0.713	0.766	0.803	0.830	0.851
1100 μM	0.319	0.426*	0.510*	0.576*	0.629	0.672	0.708	0.761	0.799	0.827	0.848
1200 μM	0.314	0.420*	0.504*	0.570*	0.624	0.667	0.703	0.757	0.795	0.824	0.845
1300 μM	0.310	0.415*	0.499*	0.565*	0.619	0.662	0.698	0.753	0.792	0.821	0.843
1400 μM	0.305	0.410*	0.493*	0.560*	0.614	0.657	0.693	0.749	0.788	0.818	0.840
1500 μM	0.302	0.406*	0.489*	0.555*	0.609	0.653	0.689	0.745	0.785	0.815	0.837
1600 μM	0.298	0.402*	0.484*	0.550*	0.604	0.649	0.685	0.741	0.782	0.812	0.835
1700 μM	0.295	0.397	0.480*	0.546*	0.600*	0.644	0.681	0.738	0.779	0.809	0.832
1800 μM	0.291	0.394	0.476*	0.542*	0.596*	0.640	0.677	0.734	0.776	0.806	0.830
1900 μM	0.288	0.390	0.472*	0.538*	0.592*	0.636	0.674	0.731	0.773	0.804	0.828
2000 μM	0.285	0.386	0.468*	0.534*	0.588*	0.633	0.670	0.728	0.770	0.801	0.826
2100 μM	0.282	0.383	0.464*	0.530*	0.584*	0.629	0.667	0.725	0.767	0.799	0.823
2200 μM	0.280	0.380	0.460*	0.526*	0.581*	0.626	0.663	0.722	0.764	0.797	0.821
2300 μM	0.277	0.376	0.457*	0.523*	0.577*	0.622	0.660	0.719	0.762	0.794	0.819
2400 μM	0.275	0.373	0.454*	0.520*	0.574*	0.619	0.657	0.716	0.759	0.792	0.817
2500 μM	0.272	0.371	0.451*	0.516*	0.571*	0.616	0.654	0.713	0.757	0.790	0.815
2600 μM	0.270	0.368	0.448*	0.513*	0.567*	0.613	0.651	0.711	0.754	0.788	0.813
2700 μM	0.268	0.365	0.445*	0.510*	0.564*	0.610	0.648	0.708	0.752	0.785	0.811
2800 μM	0.266	0.362	0.442*	0.507*	0.561*	0.607	0.645	0.705	0.750	0.783	0.810
2900 μM	0.264	0.360	0.439*	0.504*	0.558*	0.604	0.642	0.703	0.748	0.781	0.808
3000 μM	0.261	0.357	0.436*	0.501*	0.556*	0.601	0.640	0.700	0.745	0.779	0.806