A Novel Method for Detecting Terminal Glycans Using SDS-PAGE

We have developed a novel technique capable of probing terminal monosaccharides on proteins using SDS-PAGE. This technique does not require expensive equipment and is more specific than comparable methods. Additionally, it offers any laboratory the ability to gain a greater understanding of the glycan profiles of their protein of interest without significant investments.

Download A Novel Method for Detecting Terminal Glycans Using SDS-PAGE (PDF 1.7MB) to learn about how our novel technique is:

  • SENSITIVE. Labeling is through covalent bonding via click chemistry
  • INEXPENSIVE. Does not require mass spectrometry or HPLC
  • DIRECT. No requirement for releasing glycans from the protein
  • SIMPLE. Only common laboratory equipment used for Western blot is needed
  • FAST. Results can be obtained in a day
A Novel Method for Detecting Terminal Glycans Using SDS-PAGE