Webinar: Optimizing CRISPR/Cas9 Cell Line Development With Microfluidic Single-Cell Sorting
Webinar Summary
Generating validated knockout cell lines demands both precise genome editing and reliable single-cell isolation. This session presents case study data on CRISPR/Cas9 knockout generation across commercially available and immortalized primary renal epithelial cells and iPSCs, with a focus on workflow strategies that improve clonal outgrowth, viability, and throughput.
Speakers will share experimental results from the optimization and validation of ß2M knockout iPSC clones, including protein-level confirmation of successful editing. They will demonstrate how integrating advanced culture reagents with microfluidic sorting technology can reduce the time and labor associated with clonal cell line development.
What you will learn:
- Review case study data on CRISPR/Cas9 knockout generation across renal epithelial cells and iPSCs, including ß2M knockout clones validated at the protein level
- Learn strategies for improving single-cell survival, sorting accuracy, and clonal outgrowth to increase throughput and reproducibility in cell line development workflows
- Apply practical guidance for overcoming bottlenecks in clonal propagation, genomic screening, and the integration of microfluidic sorting with advanced culture media
- Gain practical strategies for improving single-cell survival during cloning using CEPT cocktail supplementation, and for selective sorting of fluorescently labelled, viable gene-edited cells with dual-laser fluorescence detection