5-Ph-IAA

Tocris Bioscience | Catalog # 7392

Selective and potent TAG Degrader for auxin-inducible degron 2 system
Tocris Bioscience

Key Product Details

Description

Selective and potent TAG Degrader for auxin-inducible degron 2 system
View all Active Degraders »

Product Description

5-Ph-IAA is a selective and potent "Bump & Hole" TAG Degrader (DC50,6h = 17.0 nM, T1/2 = 62.3 min), for use within an auxin-inducible degron (AID2) system. 5-Ph-IAA is an analog (the “Bump”) of natural auxin that induces rapid degradation of a target protein tagged with mAID F-box protein (the "Hole" or mini AID), and recruits a mutant E3 ligase complex, OsTIR1(F74G). The induced degradation is reversible 3 h after washout with fresh medium. 5-Ph-IAA shows rapid depletion of mAID-tagged nuclear and cytoplasmic proteins in proliferating and non-proliferating mammalian cells with no detectable basal degradation, lower cytotoxicity level, increased potency and faster degradation of the reporter in comparison with the conventional AID system. The AID2 system also works in budding yeast and fission yeast, and it can control protein expression in multiple mouse organs in vivo.

5-Ph-IAA-AM (Cat. No. 7893), an analog of 5-Ph-IAA, is also available.

Plasmid vectors for the AAV transfection and CRISPR-mediated knock-in of OsTIR1F74G and mAID tag are available from Addgene.

Product Specifications for 5-Ph-IAA

Molecular Weight

251.29

Formula

C16H13NO2

Storage

Store at -20°C

Purity

≥98% (HPLC)

Chemical Name

5-Phenyl-1H-indole-3-acetic acid

CAS Number

168649-23-8

PubChem ID

15207491

InChI Key

ACCCWYHBDGKDKN-UHFFFAOYSA-N

SMILES

OC(CC1=CNC2=C1C=C(C3=CC=CC=C3)C=C2)=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 25.13 100
Ethanol 25.13 100

Preparing Stock Solutions for 5-Ph-IAA

The following data is based on the product molecular weight 251.29.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 3.98 mL 19.90 mL 39.79 mL
5 mM 0.80 mL 3.98 mL 7.96 mL
10 mM 0.40 mL 1.99 mL 3.98 mL
50 mM 0.08 mL 0.40 mL 0.80 mL

Calculators

Calculate the mass, volume, or concentration required for a solution.

=
x
x
g/mol

*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and CoA (available online).

Calculate the dilution required to prepare a stock solution.

x
=
x

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background References

References are publications that support the biological activity of the product. See our Citations tab to view 9 publications citing the usage of this product.

Product Documents for 5-Ph-IAA

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for 5-Ph-IAA

For research use only

Customer Reviews for 5-Ph-IAA

There are currently no reviews for this product. Be the first to review 5-Ph-IAA and earn rewards!

Have you used 5-Ph-IAA?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card