5-TAMRA NHS

Tocris Bioscience | Catalog # 7665

Fluorescent amine-reactive dye for labeling proteins, peptides and oligonucleotides, FRET acceptor for FAM
Tocris Bioscience
Discontinued Product
7665 has been discontinued. View all Fluorescent Probes products.

Key Product Details

Description

Fluorescent amine-reactive dye for labeling proteins, peptides and oligonucleotides, FRET acceptor for FAM

Wavelength

Excitation = 546 nm, Emission = 580 nm
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Product Description

Key information:

5-TAMRA NHS is an orange fluorescent dye supplied with an NHS ester reactive group for the labeling of primary amines. Can be used for oligonucleotide labeling.

Application: Suitable for fluorescence microscopy, confocal microscopy, FRET acceptor for FAM.

Properties and Photophysical Data: Excitation and emission maxima (λ) are 546 nm and 580 nm, respectively; quantum yield = 0.1; extinction coefficient = 95,000 M-1cm-1; A280 = 0.178.

Please see the protocol for further information on protein/antibody labeling and conjugation.

It is recommended to prepare stock solutions in DMSO.

Optical Data

Emission Color Orange
Brightness
λabs 546 nm
λem 580 nm
Extinction Coefficient (ε) 95,000 M-1cm-1
Quantum Yield (φ) 0.1
Closest Laser line 532 nm
Reactive Group NHS ester
Reactivity Primary amines
Cell Permeable Yes
pH Sensitivity No
Correction Factor 280 0.178
Application Fluorescence Microscopy, Confocal Microscopy
Alternative For Alexa Fluor® 546

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Product Specifications for 5-TAMRA NHS

Molecular Weight

527.52

Formula

C29H25N3O7

Storage

Store at -20°C

Purity

≥90% (HPLC)

Chemical Name

2-[3-(Dimethylamino)-6-dimethylazaniumylidenexanthen-9-yl]-5-(2,5-dioxopyrrolidin-1-yl)oxycarbonylbenzoate

CAS Number

150810-68-7

PubChem ID

2762600

InChI Key

VWFRSNKRTNUMET-UHFFFAOYSA-N

SMILES

CN(C)C1=CC=C(C(C2=CC=C(C(ON3C(CCC3=O)=O)=O)C=C2C([O-])=O)=C(C=C/4)C(O5)=CC4=[N+](C)\C)C5=C1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

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Background References

References are publications that support the biological activity of the product.

Product Documents for 5-TAMRA NHS

Certificate of Analysis

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Product Specific Notices for 5-TAMRA NHS

For research use only

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