Acylglycerol Kinase Antibody

Novus Biologicals | Catalog # NBP1-06013

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse

Applications

Validated:

Western Blot, Peptide ELISA

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all AGK Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence CDPRKREQMLTSP corresponding to C-Terminus according to NP_060708.1.

Epitope

CDPRKREQMLTSP

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 26635614).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Acylglycerol Kinase Antibody

Western Blot: Acylglycerol Kinase Antibody [NBP1-06013]

Western Blot: Acylglycerol Kinase Antibody [NBP1-06013]

Western Blot: Acylglycerol Kinase Antibody [NBP1-06013] - Analysis of Acylglycerol Kinase using NBP1-06013 at 1ug/ml in Human Brain (Substantia nigra) lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Applications for Acylglycerol Kinase Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:16000

Western Blot

1 - 3 ug/ml
Application Notes
WB: Approx. 50 kDa band observed in human brain (substantia nigra, hippocampus) lysates (calculated MW of 47.1 kDa band according to NP_060708.1). An additional band of unknown identity was also consistently observed at 22 kDa. This band was successfully blocked by incubation with the immunizing peptide.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: AGK

Lipid kinase that can phosphorylate both monoacylglycerol and diacylglycerol to form lysophosphatidic acid (LPA) and phosphatidic acid (PA), respectively. Does not phosphorylate sphingosine. Overexpression increases the formation and secretion of LPA, resulting in transactivation of EGFR and activation of the downstream MAPK signaling pathway, leading to increased cell growth

Long Name

Acylglycerol Kinase

Alternate Names

FLJ10842, MULK

Entrez Gene IDs

55750 (Human); 69923 (Mouse)

Gene Symbol

AGK

UniProt

NP_060708.1 (Human)

Additional AGK Products

Product Documents for Acylglycerol Kinase Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Acylglycerol Kinase Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Bcl-2 Proteins and Regulators

Citations for Acylglycerol Kinase Antibody

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