alcohol dehydrogenase Antibody - BSA Free

Novus Biologicals | Catalog # NB600-912

Novus Biologicals

Key Product Details

Species Reactivity

Yeast

Applications

Western Blot, ELISA, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all alcohol dehydrogenase Primary Antibodies »

Product Specifications

Immunogen

alcohol dehydrogenase [Yeast] (Uniprot: P00330)

Reactivity Notes

Cross reactivity against alcohol dehydrogenase from other sources may occur but have not been specifically determined.

Localization

Cytoplasmic

Specificity

Recognises Alcohol Dehydrogenase. Cross reactivity against Alcohol Dehydrogenase from other sources may occur but have not been specifically determined.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Alcohol Dehydrogenase [Yeast]

Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

Scientific Data Images for alcohol dehydrogenase Antibody - BSA Free

Western Blot: alcohol dehydrogenase Antibody [NB600-912]

Western Blot: alcohol dehydrogenase Antibody [NB600-912]

Western Blot: alcohol dehydrogenase Antibody [NB600-912] - Analysis using Azide/BSA FREE version of NB600-912. Detection of yeast Alcohol Dehydrogenase. Comparison to molecular weight markers (not shown) indicates estimated molecular weights consistent with monomer, dimer and tetramer present in this preparation. The blot was incubated with a 1:500 dilution of the antibody at room temperature for 2 h followed by detection using 800 labeled Goat-a-Rabbit IgG [H&L] diluted 1:5,000 for 45 min at room temperature.
Western Blot: alcohol dehydrogenase Antibody [NB600-912]

Western Blot: alcohol dehydrogenase Antibody [NB600-912]

Western Blot: alcohol dehydrogenase Antibody [NB600-912] - Lane 1: Alcohol Dehydrogenase. Load: 50ng per lane. Primary antibody: Alcohol Dehydrogenase Antibody at 1:1,000 overnight at 4C. Secondary antibody: Peroxidase Conjugated Rabbit secondary antibody at 1:40,000 for 30 min at RT. Block: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 37kDa, 45kDa.
Western Blot: alcohol dehydrogenase Antibody [NB600-912]

Western Blot: alcohol dehydrogenase Antibody [NB600-912]

Western Blot: alcohol dehydrogenase Antibody [NB600-912] - Analysis using Biotin conjugate of NB600-912. Lane 1: Alcohol Dehydrogenase (yeast). Load: 50 ng per lane. Primary antibody: Rabbit anti-Alcohol Dehydrogenase (Yeast) Antibody Biotin Conjugated at 1:1,000 overnight at 4C. Secondary antibody: Peroxidase Streptavidin Antibody at 1:40,000 for 30 min at RT. Block: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 37 kDa, 45 kDa for Alcohol Dehydrogenase (yeast).

Applications for alcohol dehydrogenase Antibody - BSA Free

Application
Recommended Usage

ELISA

1:5000-1:20000

Immunoprecipitation

1:100

Western Blot

1:500-1:2000
Application Notes
This product has been tested by western blot and is suitable for use in ELISA and immunoprecipitation. Specific conditions for reactivity should be optimized by the end user.

Formulation, Preparation, and Storage

Purification

Multi-step

Reconstitution

Reconstitute with 100 ul deionized water (or equivalent)

Formulation

Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Format

BSA Free

Preservative

0.01% Sodium Azide

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store lyophilized antibody at 4C. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: alcohol dehydrogenase

ADH (alcohol dehydrogenase) family of proteins metabolize a variety of substances such as ethanol, retinal, other aliphatic alcohol, hydroxysteroids, and lipid peroxidation products. With the coenzyme NAD, ADH catalyzes the reversible conversion of organic alcohol to ketones or aldehydes. ADH play a major role in ethanol metabolism.

Alternate Names

ADH, alpha subunit, ADH1alcohol dehydrogenase 1A, alcohol dehydrogenase 1 (class I), alpha polypeptide, alcohol dehydrogenase 1A (class I), alpha polypeptide, Alcohol dehydrogenase subunit alpha, aldehyde reductase, EC 1.1.1, EC 1.1.1.1

Gene Symbol

ADH1A

UniProt

P00330 (Yeast)

Additional alcohol dehydrogenase Products

Product Documents for alcohol dehydrogenase Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for alcohol dehydrogenase Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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