α-Amanitin

Tocris Bioscience | Catalog # 4025

Inhibitor of RNA polymerase II
Tocris Bioscience

Key Product Details

Description

Inhibitor of RNA polymerase II
View all RNA/DNA Polymerase Inhibitors »

Product Description

α-Amanitin is an inhibitor of RNA polymerase II. Inhibits transcription in eukaryotic cells. Binds and blocks the largest subunits of RNA polymerase II, preventing new ribonucleotides from incorporating into the nascent RNA chain. Potent amatoxin.

Product Specifications for α-Amanitin

Molecular Weight

918.97

Formula

C39H54N10O14S

Storage

Store at -20°C

Purity

≥95% (HPLC)

Chemical Name

cyclo[L-Asparaginyl-4-hydroxy-L-prolyl-(R)-4,5-dihydroxy-L-isoleucyl-6-hydroxy-2-mercapto-L-tryptophylglycyl-L-isoleucylglycyl-L-cysteinyl]cyclic (4→8)-sulfide (R)-S-oxide

CAS Number

23109-05-9

PubChem ID

441541

InChI Key

CIORWBWIBBPXCG-JAXJKTSHSA-N

SMILES

O=[C@@](N[C@@](C(N[C@@H](CC2=C(S4=O)NC3=C2C=CC(O)=C3)C(NCC(N[C@]([H])5[C@@H](C)CC)=O)=O)=O)([H])[C@@H](C)[C@@H](O)CO)[C@@H]1C[C@@H](O)CN1C([C@H](CC(N)=O)NC([C@H](C4)NC(CNC5=O)=O)=O)=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
water 4.59 5

Preparing Stock Solutions for α-Amanitin

The following data is based on the product molecular weight 918.97.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.05 mM 21.76 mL 108.82 mL 217.63 mL
0.25 mM 4.35 mL 21.76 mL 43.53 mL
0.5 mM 2.18 mL 10.88 mL 21.76 mL
2.5 mM 0.44 mL 2.18 mL 4.35 mL

Calculators

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Background References

References are publications that support the biological activity of the product. See our Citations tab to view 7 publications citing the usage of this product.

Product Documents for α-Amanitin

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for α-Amanitin

For research use only

Related Research Areas

Epigenetics

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  • alpha -Amanitin was used in antibacterial resistance experiments
    Name: Anonymous
    Species: Human
    Verified Customer | Posted 02/20/2019
    alpha -Amanitin was used in antibacterial resistance experiments.We looked into RNAP translocation along the DNA without dissociating from either the template or the growing RNA product until it encounters a termination factor or signal that causes the transcription cycle end with dissociation of the transcribing complex and release of RNA polymerase for a new round of transcription.
    α-Amanitin 4025

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