Rat alpha-Smooth Muscle Actin ELISA Kit (Colorimetric)

Novus Biologicals | Catalog # NBP2-66430

Novus Biologicals
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Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and other biological fluids (100 uL)

Sensitivity

1.88 ng/mL

Assay Range

3.13 - 200 ng/mL
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Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Species

Rat

Specificity

This kit recognizes Rat ACTalpha2 in samples. No significant cross-reactivity or interference between Rat ACTalpha2 and analogues was observed.

精度

Intra-Assay Precision (Precision within an assay) CV% 5.29%

Inter-Assay Precision (Precision between assays) CV% 5.51%

Recovery for Rat alpha-Smooth Muscle Actin ELISA Kit (Colorimetric)

Recovery

84-102%

Linearity

Rat alpha-Smooth Muscle Actin ELISA Kit (Colorimetric)

Scientific Data Images for Rat alpha-Smooth Muscle Actin ELISA Kit (Colorimetric)

ELISA: Rat alpha-Smooth Muscle Actin ELISA Kit (Colorimetric) [NBP2-66430]

ELISA: Rat alpha-Smooth Muscle Actin ELISA Kit (Colorimetric) [NBP2-66430]

ELISA: Rat alpha-Smooth Muscle Actin ELISA Kit (Colorimetric) [NBP2-66430] - Standard Curve. Image from verified customer review.
Rat alpha-Smooth Muscle Actin ELISA Kit (Colorimetric)

ELISA: Rat alpha-Smooth Muscle Actin ELISA Kit (Colorimetric) [NBP2-66430] -

ELISA: Rat alpha-Smooth Muscle Actin ELISA Kit (Colorimetric) [NBP2-66430] - Image from verified customer review.
Rat alpha-Smooth Muscle Actin ELISA Kit (Colorimetric)

ELISA: Rat alpha-Smooth Muscle Actin ELISA Kit (Colorimetric) [NBP2-66430] -

ELISA: Rat alpha-Smooth Muscle Actin ELISA Kit (Colorimetric) [NBP2-66430] - Image from verified customer review.
Rat alpha-Smooth Muscle Actin ELISA Kit (Colorimetric)

ELISA: Rat alpha-Smooth Muscle Actin ELISA Kit (Colorimetric) [NBP2-66430] -

ELISA: Rat alpha-Smooth Muscle Actin ELISA Kit (Colorimetric) [NBP2-66430] - Standard Curve Reference

Kit Contents for Rat alpha-Smooth Muscle Actin ELISA Kit (Colorimetric)

  • Biotinylated Detection Ab Diluent
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate (100x)
  • Concentrated Wash Buffer (25x)
  • HRP Conjugate Diluent
  • Micro ELISA Plate (Dismountable)
  • Plate Sealer
  • Product Manual
  • Reference Standard
  • Sample Diluent
  • Stop Solution
  • Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: alpha-Smooth Muscle Actin

Actin proteins are highly conserved in eukaryotic cells and are involved in cell motility, structure and integrity. Actin and myosin are involved in a myriad of cellular processes including locomotion, secretion, cytoplasmic streaming, phagocytosis and cytokinesis. Actin filaments, along with microtubules, comprise a major component of the cytoskeleton and play essential roles in regulating cell shape and movement. Actin has been found to be expressed in at least six isoforms, each encoded by a separate gene and expressed in a developmentally regulated and tissue-specific manner. Alpha, beta and gamma actin isoforms have been identified. Alpha actins are a major constituent of the contractile apparatus, whereas beta and gamma actins are involved in the regulation of cell motility.

ACTA2 encodes alpha Smooth Muscle Actin and is also known as alpha-actin, alpha-actin-2, aortic smooth muscle or alpha smooth muscle actin (alpha-SMA, SMactin, alpha-SM-actin, ASMA). Alpha Smooth Muscle Actin is frequently used as a marker of smooth muscle differentiation and has a theoretical molecular weight of 42 kDa. Smooth muscle alpha actin is one of a few genes whose expression is predominantly expressed in vascular smooth muscle cells that is used to study myofibroblasts and fibrosis (1). Excessive accumulation of alpha smooth muscle actin-positive (ACTA2+) activated myofibroblasts is observed in Idiopathic pulmonary fibrosis (2). Expression of smooth muscle alpha actin is regulated by cell proliferation and is altered in pathological conditions including atherosclerosis and is common in metastatic cancers (1,3).

References

1. Chakraborty, R., Saddouk, F. Z., Carrao, A. C., Krause, D. S., Greif, D. M., & Martin, K. A. (2019). Promoters to Study Vascular Smooth Muscle. Arterioscler Thromb Vasc Biol, 39(4), 603-612. doi:10.1161/atvbaha.119.312449

2.El Agha, E., Moiseenko, A., Kheirollahi, V., De Langhe, S., Crnkovic, S., Kwapiszewska, G.,... Bellusci, S. (2017). Two-Way Conversion between Lipogenic and Myogenic Fibroblastic Phenotypes Marks the Progression and Resolution of Lung Fibrosis. Cell Stem Cell, 20(2), 261-273.e263. doi:10.1016/j.stem.2016.10.004

3. Chen, Y. C., Gonzalez, M. E., Burman, B., Zhao, X., Anwar, T., Tran, M.,... Kleer, C. G. (2019). Mesenchymal Stem/Stromal Cell Engulfment Reveals Metastatic Advantage in Breast Cancer. Cell Rep, 27(13), 3916-3926.e3915. doi:10.1016/j.celrep.2019.05.084

Long Name

Actin, Alpha 2, Smooth Muscle, Aorta

Alternate Names

AAT6, ACTA2, Actin alpha 2, ACTSA, ACTVS, alphaSmooth Muscle Actin, MYMY5, SMA

Gene Symbol

ACTA2

Additional alpha-Smooth Muscle Actin Products

Product Documents for Rat alpha-Smooth Muscle Actin ELISA Kit (Colorimetric)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Rat alpha-Smooth Muscle Actin ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Citations for Rat alpha-Smooth Muscle Actin ELISA Kit (Colorimetric)

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  • Rat alpha-Smooth Muscle Actin ELISA Kit (Colorimetric)
    Name: Anonymous
    Sample Tested: Serum and Lung homogenates
    Verified Customer | Posted 06/24/2023
    Rat alpha-Smooth Muscle Actin ELISA Kit (Colorimetric) NBP2-66430
  • Name: Anonymous
    Verified Customer | Posted 05/23/2023
    rat lung
    Rat alpha-Smooth Muscle Actin ELISA Kit (Colorimetric) NBP2-66430
  • Rat alpha-Smooth Muscle Actin ELISA Kit (Colorimetric)
    Name: Vishal Patel
    Sample Tested: Rat Lung Tissue
    Verified Customer | Posted 10/22/2022
    DRC
    Rat alpha-Smooth Muscle Actin ELISA Kit (Colorimetric) NBP2-66430

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FAQs for Rat alpha-Smooth Muscle Actin ELISA Kit (Colorimetric)

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    • Q: I do not see horse listed as a predicted or confirmed species. Would you be able to confirm if any of your alpha-SMA antibodies will react with equine samples?
      A: We have not directly tested equine samples nor do we have cited publications that have tested equine samples. Since alpha-Smooth Muscle Actin is a highly conserved sequence, these antibodies should react with most mammal species.
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