Bovine Annexin V - Ready-To-Use ELISA Kit (Colorimetric)
Novus Biologicals | Catalog # NBP3-54346
Key Product Details
Sample Type & Volume Required Per Well
Sensitivity
Assay Range
Product Specifications
Assay Type
Kit Type
Species
Description
Assay Length: 3 hours
精度
Intra-Assay Precision (Precision within an assay) %CV 10 (example only; lot dependent)
Inter-Assay Precision (Precision between assays) %CV 12 (example only; lot dependent)
Kit Contents for Bovine Annexin V - Ready-To-Use ELISA Kit (Colorimetric)
- Pre-coated 96T strip plate, Plate sealer for 96 wells, Standard, Standard Diluent, Detection Solution A, Detection Solution B, TMB Substrate, Stop Solution, Wash Buffer (30 x concentrate), Instruction manual
Preparation and Storage
Stability & Storage
Background: Annexin V
Alternate Names
Gene Symbol
Additional Annexin V Products
Product Documents for Bovine Annexin V - Ready-To-Use ELISA Kit (Colorimetric)
Certificate of Analysis
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Product Specific Notices for Bovine Annexin V - Ready-To-Use ELISA Kit (Colorimetric)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
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FAQs for Bovine Annexin V - Ready-To-Use ELISA Kit (Colorimetric)
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Q: I am studying apoptosis in adherent cells. Can I trypsinize the cells when using Annexin V-FITC to stain the cells?
A: Adherent cells undergoing apoptosis automatically detach from the plate and may not require trypsinization. If there are attached cells, they may be trypsinized without affecting phosphatidylserine (PS).
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Q: I do not have access to flow cytometer in my building. Can I stain for Annexin V and propidium iodide (PI), fix the cells, then analyze them in a different facility?
A: Fixing the cells can affect cell permeability, enhancing the uptake of Annexin V and PI intracellularly. As a result, the profile of fixed cells may be different from unfixed cells, and the use of appropriate controls is recommended.
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Q: I am studying apoptosis in adherent cells. Can I trypsinize the cells when using Annexin V-FITC to stain the cells?
A: Adherent cells undergoing apoptosis automatically detach from the plate and may not require trypsinization. If there are attached cells, they may be trypsinized without affecting phosphatidylserine (PS).
-
Q: I do not have access to flow cytometer in my building. Can I stain for Annexin V and propidium iodide (PI), fix the cells, then analyze them in a different facility?
A: Fixing the cells can affect cell permeability, enhancing the uptake of Annexin V and PI intracellularly. As a result, the profile of fixed cells may be different from unfixed cells, and the use of appropriate controls is recommended.